Polymerase chain reaction for detection of Mycobacterium tuberculosis.

Journal of Clinical Microbiology
U SjöbringH Miörner

Abstract

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

References

Jul 1, 1989·Molecular Microbiology·A J HanceB Gicquel
Mar 1, 1987·Journal of Bacteriology·T M Shinnick

❮ Previous
Next ❯

Citations

Dec 1, 1992·The Histochemical Journal·A M ClarkeP Quirke
Apr 1, 1992·Neuropathology and Applied Neurobiology·S Love, J A Nicoll
Jan 1, 1995·Journal of Clinical Laboratory Analysis·Z Ronai, M Yakubovskaya
Jun 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J J PalaciosJ F de Quirós
Dec 16, 1998·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·R Drut, G Quijano
Mar 21, 2002·Expert Review of Molecular Diagnostics·M Glennon, M Cormican
Aug 26, 2003·Journal of Clinical Laboratory Analysis·Sanjay K GargPrakash S Bisen
Apr 1, 1996·Critical Reviews in Clinical Laboratory Sciences·C D HeroldD A Herold
Oct 14, 1998·American Journal of Respiratory and Critical Care Medicine·S JouveshommeJ Grosset
May 30, 1998·The Tohoku Journal of Experimental Medicine·B YangS Kohno
Jan 1, 1995·The Journal of Laryngology and Otology·R G Williams, T Douglas-Jones
Sep 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M Fauville-DufauxJ Content
Feb 1, 1992·Acta Paediatrica·M NaritaH Kobayashi
Dec 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A B AndersenN G Stoker
Oct 24, 2001·Scandinavian Journal of Infectious Diseases·J HondaK Oizumi
Jan 1, 1996·Scandinavian Journal of Infectious Diseases·S E HoffnerG Kallenius
Aug 1, 1992·Letters in Applied Microbiology·G RodrigoS B Svenson
May 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Soini, M K Viljanen
Oct 3, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P AlonsoA Rodríguez Torres
Oct 1, 1993·Journal of Dairy Science·P C Vasavada
May 9, 2002·British Medical Bulletin·Steven Myint
Jul 1, 2000·Indian Journal of Clinical Biochemistry : IJCB·S KamatchiammalV Sritharan
Apr 1, 1993·Journal of Clinical Pathology·K Y YuenM H Ng
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·A K BejR M Atlas
Aug 5, 2009·Molecular Diagnosis & Therapy·Seetha V BalasinghamTone Tønjum
Mar 7, 2002·Scandinavian Journal of Infectious Diseases·M C RajoF Pardo
Apr 6, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M C AntognoliT Keefe
Oct 3, 2019·Biosensors·Faye M Walker, Kuangwen Hsieh
Mar 1, 1997·Clinics in Chest Medicine·L Heifets
Jun 7, 2003·Journal of Clinical Microbiology·Masao FukushimaRyuji Kawaguchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.