PMID: 8610949May 1, 1996Paper

Polymerase chain reaction for the diagnosis of HIV infection in adults. A meta-analysis with recommendations for clinical practice and study design

Annals of Internal Medicine
D K OwensJ S Schwartz

Abstract

To do a meta-analysis of studies that have evaluated the sensitivity and specificity of polymerase chain reaction (PCR) assay for the diagnosis of human immunodeficiency virus (HIV) infection in adults. Evaluating the performance of PCR is difficult because in certain clinical situations, the sensitivity or specificity of PCR may exceed those of the current reference standard tests (enzyme immunoassay followed by confirmatory Western blot analysis). Therefore, an additional goal was to develop recommendations for 1) the design of future evaluative studies of PCR and 2) the use of PCR in persons with suspected HIV infection. Studies published between 1988 and 1994 that were identified in a search of 17 computer databases, including MEDLINE, and abstracts identified from conference proceedings. Studies were included if DNA amplification by PCR was done on peripheral blood mononuclear cells from adults. Ninety-six studies met the inclusion criteria. Data were extracted independently by two reviewers. Study design was assessed independently by two investigators blinded to study results. Reported sensitivities for PCR range from 10% to 100%, and specificities range from 40% to 100%. A summary receiver-operating characteristic curve ...Continue Reading

Citations

Mar 3, 2004·The Annals of Pharmacotherapy·Brian J DonovanChristopher D Pilcher
Oct 17, 1996·The New England Journal of Medicine·M CorbellinoC Parravicini
Feb 20, 2015·Frontiers in Public Health·Patricia Goodson
Jun 21, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Loukia S PoulouLoukas Thanos
Feb 10, 2006·Clinical and Vaccine Immunology : CVI·Elias Zintzaras, Anastasios E Germenis
Jul 1, 1997·Medical Decision Making : an International Journal of the Society for Medical Decision Making·D K OwensR F Nease
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis
Jul 25, 2000·Clinical Microbiology Reviews·E M BeltramiM E Chamberland
Sep 25, 2002·Clinics in Laboratory Medicine·Joseph A DeSimone, Roger J Pomerantz
Feb 20, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Peter Rohner, Raymond Auckenthaler

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