PMID: 8939053Dec 1, 1996Paper

Polymerase chain reaction-identification of Dirofilaria repens and Dirofilaria immitis

Parasitology
G FaviaM Coluzzi

Abstract

On the basis of known DNA sequences of Dirofilaria repens and D. immitis we designed specific primers for the amplification by Polymerase Chain Reaction (PCR) of the DNA from the two species. The PCR-based identification was found to be unambiguous and allowed specific diagnosis of microfilariae in blood samples, of developing larvae in the mosquito vector and of immature adults in bioptic material, overcoming the serious constraints of the morphological separation of these filarial parasites at the pre-adult stages. The technique was found to be very sensitive and applicable to samples stored either dry or in various preservation media, with the exception of formalin. The reliable identification of D. repens and D. immitis from bioptic material is expected to greatly enhance the chances of detecting human infections and to further clarify the role of the two parasites as pathogens of man. The possibility of routine identification of developing larvae in the vector will substantially improve the perspectives for epidemiological investigations, particularly in Southern European regions, such as Italy, where the two nematode species are largely sympatric.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C B PooleL A McReynolds
Oct 1, 1991·Annals of Tropical Medicine and Parasitology·G CancriniM Coluzzi
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·G FaviaC Louis
Jan 1, 1996·The American Journal of Tropical Medicine and Hygiene·K SiridewaN V Chandrasekharan

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Citations

Jan 20, 2004·Veterinary Parasitology·G CancriniM Pietrobelli
Aug 26, 1999·Parasitology Today·A MuroF Simón
Aug 11, 2000·Veterinary Parasitology·G CancriniF Simón
Nov 5, 2003·Veterinary Parasitology·Guido FaviaLuca Rossi
Aug 24, 1999·Veterinary Parasitology·R B Gasser
Aug 6, 2000·Diagnostic Microbiology and Infectious Disease·G Favia
Mar 28, 2001·Parasitology International·N VakalisN C Vamvakopoulos
Nov 6, 2007·Vector Borne and Zoonotic Diseases·Rodrigo MorchónFernando Simón
Feb 12, 2008·Vector Borne and Zoonotic Diseases·Marta MagiGabriella Cancrini
Jun 26, 2010·Vector Borne and Zoonotic Diseases·Johny Antonio Caro-GonzalezCarlos Humberto Sauri-Arceo
Sep 18, 2010·Vector Borne and Zoonotic Diseases·Fernando O AvellisFrancesco Rivasi
Nov 7, 2012·Vector Borne and Zoonotic Diseases·Aleksandar TasićGabriella Cancrini
Jan 15, 1999·The Journal of Small Animal Practice·W P BredalL S Mansfield
Sep 28, 2005·BMC Infectious Diseases·Paola ScaramozzinoGabriella Cancrini
Feb 7, 2014·Malaria Journal·Jeffrey R PowellVincenzo Petrarca
Mar 21, 2007·Emerging Infectious Diseases·Laura H KramerFernando Simón
Jan 10, 2002·Diagnostic Cytopathology·W K NgJames H B Kong
Jul 16, 2011·Parasitology Research·Nikola PantchevViktor Dyachenko
May 15, 2015·Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere·V HärtwigV Dyachenko
Jul 10, 2009·Journal of Cutaneous Pathology·Konstantina TzanetouKonstantina Fragia
Dec 4, 2003·Medical and Veterinary Entomology·G CancriniP Scaramozzino
Aug 16, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Francesco RivasiSilvio Pampiglione
Jul 12, 2011·Zoonoses and Public Health·S TasićG Cancrini
Jul 22, 2006·Veterinary Parasitology·Maurizio CasiraghiClaudio Genchi
Nov 18, 2005·Veterinary Parasitology·Mark RishniwJose Luis Dominguez Alpizar
Sep 3, 2003·The Journal of Veterinary Medical Science·Tadashi ItagakiShiro Chinone
Apr 18, 2015·Parasites & Vectors·Cátia Alexandra Costa FerreiraAntónio Paulo Gouveia de Almeida
Jan 18, 2012·Acta Tropica·Maria Stefania LatrofaDomenico Otranto
Feb 17, 2015·TheScientificWorldJournal·Sonjoy Kumar BorthakurPrabhat Chandra Sarmah
Jun 3, 2006·Journal of Medical Entomology·G CancriniM C Prati
Feb 11, 2014·BMC Infectious Diseases·Suzana A Tasić-OtaševićGabriella A Cancrini
Oct 4, 2005·Veterinary Parasitology·F SimónJ R Martín-Pacho
Dec 10, 2016·Veterinary Clinical Pathology·Simone ManzocchiNikola Pantchev
Feb 19, 2014·Parasite : Journal De La Société Française De Parasitologie·Ana Luísa VieiraJuan Gestal

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