Polymerase chain reaction mediated identification of Streptococcus uberis and Streptococcus parauberis using species-specific sequences of the genes encoding superoxide dismutase A and chaperonin 60*

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
J AlberMichael Zschöck

Abstract

Streptococcus uberis, a well-known bacterial pathogen associated with bovine mastitis, appears to be biochemically and serologically almost indistinguishable from the closely related species Streptococcus parauberis. In the present study, species-specific oligonucleotide primers were designed using internal parts of the genes sodA, encoding superoxide dismutase A, and cpn60 encoding chaperonin 60 of S. uberis and S. parauberis, respectively. The two oligonucleotide primer pairs allowed a rapid and reliable PCR-mediated identification and differentiation of both species. These studies, performed with S. uberis and S. parauberis reference cultures and clinical isolates from routine diagnostics, revealed that the occurrence of S. parauberis as causative agent of bovine mastitis appears to be rare. In addition the sodA and cpn60 sequence data confirmed that both species could taxonomically be classified to the pyogenic group of genus Streptococcus.

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Citations

Jun 19, 2013·Veterinary Microbiology·Ashley N HainesWolfgang K Vogelbein
Nov 23, 2006·BMC Veterinary Research·Florence B GilbertBernard Poutrel
May 9, 2007·Current Microbiology·Mariana Sá e SilvaAgueda Castagna de Vargas
Oct 28, 2014·Journal of Fish Diseases·T L NguyenB Austin
Aug 25, 2011·Research in Veterinary Science·Acácia Orieth EliasHelio Langoni
May 7, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M YounanC Lämmler
May 7, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·G DenamielE Gentilini
Jul 8, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L K FoxA Britten
Apr 12, 2016·Journal of Dairy Science·J B W J CornelissenUNKNOWN 1Health4Food—Dutch Mastitis Diagnostics Consortium
Sep 27, 2018·International Journal of Veterinary Science and Medicine·Amr El-SayedHugo Castañeda Vázquez
Dec 31, 2020·Veterinary Medicine International·Virginia E SherwinSharon A Egan

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