PMID: 9192391May 1, 1997Paper

Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation

Obesity Research
W K ChungR L Leibel

Abstract

Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C-->T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A-->C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.

References

Oct 1, 1995·The Journal of Clinical Investigation·D S WeigleJ L Kuijper
Jul 28, 1995·Science·M A PelleymounterF Collins
Dec 25, 1994·Nucleic Acids Research·O Grau, R Griffais
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·N LevinF de Sauvage

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Citations

Mar 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·György BaffyBradford B Lowell
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·E IoffeJ M Friedman
Jul 26, 2008·PLoS Genetics·Marija Dokmanovic-ChouinardRudolph L Leibel
Nov 16, 2013·PloS One·Alton G SutterKenneth D Chavin
Feb 15, 2008·Journal of Lipid Research·Angie T Oler, Alan D Attie
Sep 19, 2018·The Journal of Clinical Investigation·Hu HuangYoung-Bum Kim
Oct 25, 2008·Obesity·Justin D EllettDemetri D Spyropoulos
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K SchäferD J Loskutoff
Nov 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lutz BüngerJohn R Speakman
Sep 12, 2019·Scientific Reports·Aykut Göktürk ÜnerYoung-Bum Kim
May 12, 1998·The American Journal of Physiology·W K ChungR L Leibel

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