PMID: 4009443Mar 1, 1985Paper

Polymeric dyes: useful nonabsorbable reference markers for intestinal perfusion studies in animals

Journal of Pharmaceutical Sciences
J L DupasA F Hofmann

Abstract

The properties of two polymeric dyes were examined to determine their suitability as nonabsorbable reference markers for intestinal perfusion studies. The first dye was a violet anthroquinone dye, R-478, named polyporphyre; the second was a blue anthroquinone dye, R-411, named polycyanine. The dyes could be determined spectrophotometrically over a concentration range of 0.03-5 microM (1-200 micrograms/mL) and had no appreciable osmotic effect. They were light stable and their absorption spectra did not change in solution over a pH range of 1-11. The dyes had a molecular weight of approximately 40,000 by gel permeation chromatography; they did not appear to have hydrophobic binding sites because they did not bind added dihydroxy bile acid or fatty acid anions. Determination of the dyes was not influenced by the presence of bilirubin when the latter was present at concentrations reported to occur in small intestinal content during digestion. The dyes did not interfere with an endpoint enzymatic determination of bile acids. The dyes caused little quenching of 3H- or 14C-radioactivity in a toluene-based liquid scintillation cocktail. Dyes were easily removed from aqueous solution by passage over a large-pore anion exchange column o...Continue Reading

References

Jun 1, 1979·Food and Cosmetics Toxicology·P D WalsonT M Parkinson
Aug 1, 1978·Food and Cosmetics Toxicology·J P BrownC M Bakner
Oct 1, 1969·Journal of Pharmaceutical Sciences·J T DoluisioJ V Swintosky
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Oct 1, 1957·The Journal of Clinical Investigation·B BORGSTROMJ SJOVALL
Oct 8, 1962·Biochimica Et Biophysica Acta·J P HUMMEL, W J DREYER

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Citations

Jan 1, 1991·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M PlauthF Hartmann
Jun 1, 1995·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A RühlM Wienbeck
Aug 21, 2003·Journal of Agricultural and Food Chemistry·Zhaohui ZhaoHiroo Sanada

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