Polymerization pathway of mammalian nonmuscle myosin 2s

Proceedings of the National Academy of Sciences of the United States of America
Xiong LiuEdward D Korn

Abstract

The three mammalian nonmuscle myosin 2 (NM2) monomers, like all class 2 myosin monomers, are hexamers of two identical heavy (long) chains and two pairs of light (short) chains bound to the heavy chains. The heavy chains have an N-terminal globular motor domain (head) with actin-activated ATPase activity, a lever arm (neck) to which the two light chains bind, and a coiled-coil helical tail. Monomers polymerize into bipolar filaments, with globular heads at each end separated by a bare zone, by antiparallel association of their coiled-coil tails. NM2 filaments are highly dynamic in situ, frequently disassembling and reassembling at different locations within the cell where they are essential for multiple biological functions. Therefore, it is important to understand the mechanisms of filament polymerization and depolymerization. Monomers can exist in two states: folded and unfolded. It has been thought that unfolded monomers form antiparallel dimers that assemble into bipolar filaments. We now show that polymerization in vitro proceeds from folded monomers to folded antiparallel dimers to folded antiparallel tetramers that unfold forming antiparallel bipolar tetramers. Folded dimers and tetramers then associate with the unfolded...Continue Reading

References

Oct 1, 1975·The Journal of Cell Biology·R Niederman, T D Pollard
Feb 1, 1988·Journal of Muscle Research and Cell Motility·R A Cross
Nov 20, 1987·Journal of Molecular Biology·J Kendrick-JonesS Citi
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K M TrybusS Lowey
Mar 21, 2000·Biochimica Et Biophysica Acta·J R Sellers
Jan 5, 2005·Biochemistry·Takashi NakasawaMichio Yazawa
Aug 21, 2007·Journal of Molecular Biology·Stan A BurgessPeter J Knight
Oct 24, 2009·Nature Reviews. Molecular Cell Biology·Miguel Vicente-ManzanaresAlan Rick Horwitz
Sep 5, 2013·Bioarchitecture·Natalya G Dulyaninova, Anne R Bresnick
Sep 28, 2013·The Journal of Biological Chemistry·Neil BillingtonJames R Sellers
Feb 7, 2015·Experimental Cell Research·Jordan R Beach, John A Hammer
Jan 24, 2017·Nature Cell Biology·Shiqiong HuAlexander D Bershadsky
Jul 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Xiong LiuEdward D Korn
Mar 20, 2018·The Journal of Biological Chemistry·Hoku West-FoyleDouglas N Robinson

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Citations

Nov 9, 2018·Journal of Cell Science·Viktoria WollrabGijsje H Koenderink
May 22, 2019·Biomolecules·Joana LeiteAna Xavier Carvalho
Jun 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiong LiuEdward D Korn
Mar 18, 2019·Biochemistry. Biokhimii︠a︡·M S Shutova, T M Svitkina
Feb 20, 2021·The Journal of General Physiology·Lu WangChun Y Seow

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