Polymerized laminin-332 matrix supports rapid and tight adhesion of keratinocytes, suppressing cell migration.

PloS One
Yoshinobu KariyaKaoru Miyazaki

Abstract

Laminin-332 (α3ß3γ2) (Lm332) supports the stable anchoring of basal keratinocytes to the epidermal basement membrane, while it functions as a motility factor for wound healing and cancer invasion. To understand these contrasting activities of Lm332, we investigated Lm332 matrices deposited by normal human keratinocytes and other Lm332-expressing cell lines. All types of the cells efficiently deposited Lm332 on the culture plates in specific patterns. On the contrary, laminins containing laminin ß1 and/or γ1 chains, such as Lm511 and Lm311, were not deposited on the culture plates even if secreted into culture medium. The Lm332 deposition was not inhibited by function-blocking antibodies to the α3 and α6 integrins but was inhibited by sodium selenate, suggesting that sulfated glycosaminoglycans on cell surface, e.g. heparan sulfate proteoglycans, might be involved in the process. HEK293 cells overexpressing exogenous Lm332 (Lm332-HEK) almost exclusively deposited Lm332 on the plates. The deposited Lm332 matrix showed a mesh-like network structure as analyzed by electron microscopy, suggesting that Lm332 was highly polymerized. When biological activity was analyzed, the Lm332 matrix rather suppressed the migration of keratinocyte...Continue Reading

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Citations

Apr 15, 2014·Experimental Cell Research·Yoshiaki HirakoKatsushi Owaribe
Dec 11, 2014·Advances in Wound Care·Leeni KoivistoHannu Larjava
Feb 15, 2014·Advances in Wound Care·Susan W VolkArdeshir Bayat
Nov 26, 2015·Human Molecular Genetics·Machiko NishimuraHiroshi Shimizu
Mar 17, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Yong LiShu-Guang Wang
Sep 17, 2013·Pigment Cell & Melanoma Research·Rong ZhangDouglas E Brash
Jul 15, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Mariáurea Matias SarandyReggiani Vilela Gonçalves
Sep 1, 2016·Annual Review of Cell and Developmental Biology·Veronika Te BoekhorstPeter Friedl
Jul 23, 2013·The Journal of Clinical Investigation·Alexander NyströmLeena Bruckner-Tuderman
Dec 29, 2013·American Journal of Physiology. Renal Physiology·Soundarapandian VijayakumarDarren P Wallace

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Methods Mentioned

BETA
Enzyme-linked immunosorbent assay
ELISA
transmission electron microscopy
electron microscopy

Software Mentioned

NIH image

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