Polymicrobial periodontal disease triggers a wide radius of effect and unique virome.

NPJ Biofilms and Microbiomes
Li GaoYvonne L Kapila

Abstract

Periodontal disease is a microbially-mediated inflammatory disease of tooth-supporting tissues that leads to bone and tissue loss around teeth. Although bacterially-mediated mechanisms of alveolar bone destruction have been widely studied, the effects of a polymicrobial infection on the periodontal ligament and microbiome/virome have not been well explored. Therefore, the current investigation introduced a new mouse model of periodontal disease to examine the effects of a polymicrobial infection on periodontal ligament (PDL) properties, changes in bone loss, the host immune response, and the microbiome/virome using shotgun sequencing. Periodontal pathogens, namely Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum were used as the polymicrobial oral inoculum in BALB/cByJ mice. The polymicrobial infection triggered significant alveolar bone loss, a heightened antibody response, an elevated cytokine immune response, a significant shift in viral diversity and virome composition, and a widening of the PDL space; the latter two findings have not been previously reported in periodontal disease models. Changes in the PDL space were present at sites far away from the site of insult, indicat...Continue Reading

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Citations

Jan 21, 2021·Clinical Oral Investigations·Moan Jéfter Fernandes CostaRuthineia Diogenes Alves Uchoa Lins
Dec 15, 2020·Journal of Periodontal Research·Ryutaro KurajiYukihiro Numabe
Mar 30, 2021·Computational and Structural Biotechnology Journal·Allan Radaic, Yvonne L Kapila
May 6, 2021·Food & Function·Jhonatan de Souza CarvalhoLuís Carlos Spolidorio
Sep 1, 2021·Periodontology 2000·April MartínezYvonne L Kapila

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Methods Mentioned

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16S sequencing
enzyme-linked immunosorbent assay
X-ray

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