Polymorphic and tissue-specific imprinting of the human Wilms tumor gene, WT1

The Japanese Journal of Human Genetics
K NishiwakiM Ishikawa

Abstract

We previously demonstrated maternal monoallelic expression of the Wilms tumor suppressor gene, WT1, in about half of pre-term placental villus and fetal brain tissues examined. There were two alternative explanations for this pattern of the WT1 expression, i.e., an imprinting polymorphism vs. a developmental stage-dependent switching from monoallelic to biallelic expression of the gene. To investigate these possibilities, we examined WT1 expression in a larger number of villus samples (46 samples) with gestational ages ranging from 4 to 21 weeks, using reverse transcriptase-based polymerase chain reaction (RT-PCR) to amplify the sequences for polymorphic sites in the 3'-untranslated region (UTR) of WT1. Maternal monoallelic expression was observed in 7 (39%) of 18 samples informative for the polymorphism, while the expression of the remaining 11 samples was biallelic. In addition, there was no correlation between expression patterns and gestational ages of the samples. The results indicate that the pattern of expression (monoallelic vs. biallelic) is polymorphic. The expression patterns were also studied in five different organs from a 21-week-old fetus, showing monoallelic expression only in the placenta and biallelic expressi...Continue Reading

References

Apr 1, 1992·Nature Genetics·Y Zhang, B Tycko
Apr 1, 1992·Genomics·M GesslerG A Bruns
Mar 11, 1991·Nucleic Acids Research·P R Hoban, A M Kelsey
Feb 22, 1991·Cell·T M DeChiaraA Efstratiadis
Mar 1, 1994·Nature Genetics·Y JinnoN Niikawa
Apr 1, 1994·Nature Genetics·H SuzukiT Takahashi
May 1, 1993·Nature Genetics·N GiannoukakisC Polychronakos
Nov 25, 1993·Nature·M A Surani
Dec 15, 1993·Biochemical and Biophysical Research Communications·Y XuC Polychronakos
Apr 22, 1993·Nature·S RainierA P Feinberg
Jun 1, 1996·Human Molecular Genetics·I HatadaT Mukai

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Citations

Apr 2, 2013·The Journal of Clinical Investigation·Athar AzizAnthony R Green
Mar 4, 2000·Environmental Health Perspectives·S K Murphy, R L Jirtle
Oct 2, 2004·Human Molecular Genetics·Takeshi HirotaKenji Otsubo
Jan 19, 2008·American Journal of Medical Genetics. Part a·Timothy AntoniusArno van Heijst
Apr 6, 1999·Japanese Journal of Cancer Research : Gann·Y OjiH Sugiyama
May 26, 2007·Expert Reviews in Molecular Medicine·Suzie Ariyaratana, David M Loeb
Dec 17, 2008·PloS One·Marta Gómez-NuñezDavid A Scheinberg
Dec 21, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·S ApostolidouG E Moore
Jul 11, 2001·Genome Génome / Conseil National De Recherches Canada·A K NaumovaK Morgan
Jul 9, 2013·International Journal of Cancer. Journal International Du Cancer·Konstantina BourkoulaMin Li-Weber
Dec 18, 2013·Immunological Reviews·Ingunn M StromnesPhilip D Greenberg
Oct 12, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Daisuke KobayashiYuichi Sugiyama
Mar 30, 1999·Experimental Cell Research·R L Jirtle
Aug 7, 2003·Genome Research·H Shuen LoMaxwell P Lee

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