Polymorphic Chromosomes Bearing the Tox2 Locus in Cochliobolus carbonum Behave as Homologs during Meiosis.

Applied and Environmental Microbiology
S R Canada, L D Dunkle

Abstract

The HTS1 gene in the Tox2 locus of the fungal pathogen Cochliobolus carbonum race 1 is required for synthesis of a host-selective phytotoxin and for increased virulence on susceptible genotypes of maize. The locus is present in race 1 isolates but absent from isolates of the other races, which do not produce the toxin. By pulsed-field gel electrophoresis and Southern analysis with HTS1 sequences and chromosome-specific markers, the HTS1 gene was detected on a 4-Mb chromosome in one group of isolates and on a 2.3-Mb chromosome in another group, which lacked the 4-Mb chromosome. A chromosome-specific marker from C. heterostrophus hybridized to a 2.3-Mb chromosome in non-toxin-producing isolates and in toxin-producing isolates, including those with a 4-Mb chromosome. A marker from C. carbonum hybridized to the 4-Mb chromosome, but in isolates lacking the 4-Mb chromosome, this marker hybridized to a smaller, 2.0-Mb chromosome. Thus, the Tox2 locus is on different chromosomes in different groups of race 1 isolates. Single ascospore progeny from crosses between isolates having HTS1 on different chromosomes were analyzed for toxin-producing ability, virulence, and the presence and chromosomal location of HTS1. All progeny produced HC ...Continue Reading

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Citations

Nov 28, 2001·Applied and Environmental Microbiology·A C ChurchillV Macko
Feb 3, 2007·Natural Product Reports·Richard N Strange
Feb 3, 2000·Molecular Plant-microbe Interactions : MPMI·J W PitkinJ D Walton
Jul 31, 2002·Annual Review of Phytopathology·Thomas J WolpertLynda M Ciuffetti
Feb 28, 2002·Fungal Genetics and Biology : FG & B·Joong-Hoon AhnJonathan D Walton

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