Polymorphism at donkey β-lactoglobulin II locus: identification and characterization of a new genetic variant with a very low expression

Amino Acids
Andrea CriscioneDonata Marletta

Abstract

In the last years, donkey milk had evidenced a renewed interest as a potential functional food and a breast milk substitute. In this light, the study of the protein composition assumes an important role. In particular, β-lactoglobulin (β-LG), which is considered as one of the main allergenic milk protein, in donkey species consists of two molecular forms, namely β-LG I and β-LG II. In the present research, a genetic analysis coupled with a proteomic approach showed the presence of a new allele, here named F, which is apparently associated with a null or a severely reduced expression of β-LG II protein. The new β-LG II F genetic variant shows a theoretical average mass (Mav) of 18,310.64 Da, a value practically corresponding with that of the variant D (∆mass < 0.07 Da), but differs from β-LG II D for two amino acid substitutions: Thr100 (variant F) → Ala100 (variant D) and Thr118 (variant F) → Met118 (variant D). Proteomic investigation of the whey protein fraction of an individual milk sample, homozygous FF at β-LG II locus, allowed to identify, as very minor component, the new β-LG II F genetic variant. By MS/MS analysis of enzymatic digests, the sequence of the β-LG II F was characterized, and the predicted genomic data confi...Continue Reading

References

Dec 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A ContiG Braunitzer
Apr 11, 2000·Journal of the American Society for Mass Spectrometry·D M HornF W McLafferty
Aug 17, 2000·Journal of Protein Chemistry·M HerrouinJ Léonil
Dec 19, 2002·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Jean-Michel Wal
Mar 23, 2007·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti
May 23, 2009·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti
Jul 7, 2009·Analytical and Bioanalytical Chemistry·Patrizia RestaniAlessandro Fiocchi
Oct 29, 2009·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Dec 29, 2009·Frontiers in Bioscience (Elite Edition)·Enrico BertinoAmedeo Conti
Jan 25, 2011·Journal of Dairy Science·C BallabioA M Caroli
Nov 16, 2011·Journal of the Science of Food and Agriculture·Elinor MedhammarBarbara Burlingame
Dec 15, 2011·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Susan Waserman, Wade Watson
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Rosaria SalettiSalvatore Foti
Feb 5, 2013·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Apr 1, 1995·Journal of the American Society for Mass Spectrometry·M W SenkoF W McLaffertycor
Jul 2, 2014·Veterinaria Italiana·Silvia VincenzettiPaolo Polidori
Sep 18, 2014·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Mar 16, 2017·BMC Genomics·Christina McCarthyLuis Diambra
Aug 9, 2017·Food Research International·Vincenzo CunsoloSalvatore Foti

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Methods Mentioned

BETA
PCR
electrophoresis
Fusion Tribrid

Software Mentioned

SeqScape
Sequencing Analysis
MEGA
BLASTN
General Protein Mass Analysis for Windows
Oligo
Xcalibur
PEAKS
Xcalibur Xtract

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