Polymorphism identification in BMP15 and GDF9 genes and their association with egg production in chickens

British Poultry Science
H Y HuangQ B Wang

Abstract

1. Mutations in growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are significantly associated with reproductive performance in mammals and the objective of the present study was to identify polymorphic sites and elucidate the association between genotypes in BMP15 and GDF9 and egg production. 2. Polymorphisms in BMP15 exon1 and GDF9 exon2 were detected by DNA sequencing and PCR-RFLP. Three SNPs were detected in each of BMP15 (A111G, C231T and C34T) and GDF9 (G593A, T824C and C896T). C34T leads to the substitution of Leu by Phe, which was predicted to affect protein function. 3. Results of the association analysis indicated that C34T had an effect on total egg production at 300 d of age (EN) and age at first laying (AFE). G593A affected EN and both C231T and C896T influenced AFE. The TGC1TGC1 diplotype in BMP15 had the highest EN. 4. In conclusion, EN may be significantly improved by marker-assisted selection of the BMP15 genotypes in maternal lines of Shaobo hens.

References

Mar 20, 2001·American Journal of Human Genetics·M StephensP Donnelly
Jul 24, 2002·Biology of Reproduction·Ursula A VittAaron J W Hsueh
Dec 6, 2002·Endocrine Reviews·Hua ChangMartin M Matzuk
May 27, 2005·Biology of Reproduction·Stephanie A Pangas, Martin M Matzuk
Sep 7, 2006·The Journal of Clinical Endocrinology and Metabolism·James S PalmerGrant W Montgomery
Sep 1, 2007·The Journal of Endocrinology·S ElisP Monget

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Methods Mentioned

BETA
gene knockout
PCR
Digestion
electrophoresis

Software Mentioned

SIFT
Mwo
PHASE
SAS

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