PMID: 6401319Jan 1, 1983Paper

Polymorphism in anti-phosphocholine antibodies reflecting evolution of immunoglobulin families

The Journal of Experimental Medicine
S H ClarkeS Rudikoff

Abstract

Complete variable (V) region amino acid sequences were determined for four heavy (H) and one light (L) chain from C57BL phosphocholine (PC)-binding monoclonal antibodies. Additional NH2-terminal sequences were obtained from H and L chains of C57BL and CBA/J origin. When these V regions were compared with previously reported anti-PC sequences, a number of observations could be made regarding the function and evolution of L and H chain segments used in these antibodies. (a) L and H chain V segments are remarkably conserved in these inbred strains, although there has been an accumulation of point mutations identifying apparently allelic forms of VK and VH. (b) Mice of each genotype use the same three VK segments in combination with a single VH segment to produce most anti-PC antibodies. An exception has been noted that indicates the occasional use of a second VH gene segment. (c) Multiple, different DH regions are used by mice of each strain, which suggests that the DH segment sequence plays no critical role in either antigen binding or VH-VL pairing. Furthermore, the DH segments and their corresponding gene families appear to be highly conserved in the inbred strains studied. (d) Most PC-binding antibodies use the JH1 joining seg...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E E MaxP Leder
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E A Robinson, E Appella
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M VranaM Potter
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Rudikoff, M Potter
Feb 1, 1977·Analytical Biochemistry·C L ZimmermanJ J Pisano
Jun 22, 1971·Biochemistry·S H Bridges, J R Little
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M SegalD R Davies
Feb 29, 1972·Biochemistry·B Chesebro, H Metzger
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F W Alt, D Baltimore
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S RudikoffM Potter
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N M Gough, O Bernard
Jan 1, 1982·The Journal of Experimental Medicine·Y Kurosawa, S Tonegawa
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S H ClarkeS Rudikoff
Dec 1, 1981·The Journal of Experimental Medicine·M PawlitaM Potter

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Citations

Jan 1, 1984·Immunogenetics·A R Brown, P D Gottlieb
Jan 1, 1988·Immunogenetics·A Tutter, R Riblet
Jan 1, 1989·Immunogenetics·G KelsoeD H Schulze
Jan 1, 1996·Journal of Clinical Immunology·B ZhengS Han
May 1, 1987·Molecular Immunology·A C Buenafe, M B Rittenberg
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S RudikoffM Heller
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R I NearM L Gefter
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M A BehlkeD Y Loh
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K MeekJ D Capra
Nov 1, 1983·The Journal of Experimental Medicine·S RudikoffM Potter
Mar 1, 1984·The Journal of Experimental Medicine·S H Clarke, S Rudikoff
Dec 1, 1985·The Journal of Experimental Medicine·R M PerlmutterL Hood
Sep 1, 1987·The Journal of Experimental Medicine·M HellerS Rudikoff
Oct 1, 1988·The Journal of Experimental Medicine·S E FergusonB A Osborne
Jul 11, 1992·The Journal of Experimental Medicine·J HackettU Storb
Oct 1, 1988·Immunological Reviews·O Mäkelä, M Kaartinen
Jul 5, 2016·Fungal Genetics and Biology : FG & B·Kaitlin J Fisher, Gregory I Lang
Oct 20, 1989·Journal of Molecular Biology·A Miller, J A Glasel
Jan 1, 1992·International Reviews of Immunology·A J Feeney
Nov 1, 1991·European Journal of Immunology·M KaartinenO Mäkelä
Jul 1, 1989·European Journal of Immunology·C A PennellS H Clarke
Feb 1, 1985·European Journal of Immunology·I ToddM B Rittenberg

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