Polymorphism in microRNA-binding site in HNF1B influences the susceptibility of type 2 diabetes mellitus: a population based case-control study

BMC Medical Genetics
Naoki GodaKimiko Yamakawa-Kobayashi

Abstract

Recent genome-wide association studies (GWAS) have identified many SNPs associated with type 2 diabetes mellitus (T2DM). However, the functional roles for most of the SNPs have not been elucidated. MicroRNAs (miRNAs) are key regulators of gene expression involved in the development and progression of various diseases including T2DM. In this study, we investigated whether commonly occurring SNPs modulate miRNA-directed regulation of gene expression, and whether such SNPs in miRNA-binding sites are associated with the susceptibility for T2DM. Genotypes of eleven 3' untranslated region (UTR) SNPs of seven susceptibility genes for T2DM were determined in 353 T2DM patients and 448 control subjects. In addition, the interactions of miRNAs with the 3'UTR in the hepatocyte nuclear factor 1β (HNF1B) gene were investigated using luciferase reporter assays. One 3'UTR SNP (rs2229295) in the HNF1B gene was significantly associated with T2DM, and the frequency of an A allele (rs2229295) in T2DM patients was decreased compared with that in controls. Luciferase reporter assays showed that the SNP (rs2229295) altered the binding of two miRNAs (hsa-miR-214-5p and hsa-miR-550a-5p). We have detected the interactions of hsa-miR-214-5p/hsa-miR-550a-...Continue Reading

References

Nov 24, 1999·Mechanisms of Development·C CoffinierM Yaniv
Sep 29, 2001·The New England Journal of Medicine·S S FajansK S Polonsky
Feb 28, 2004·Science·Rohit N Kulkarni, C Ronald Kahn
Feb 28, 2004·Science·Duncan T OdomRichard A Young
Jan 22, 2005·Science·Stephen O'RahillyNicholas J Wareham
Apr 13, 2005·Lancet·Michael StumvollTimon W van Haeften
Jun 3, 2005·Journal of Medical Genetics·E L EdghillA T Hattersley
May 3, 2006·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D BodhiniV Mohan
Nov 21, 2007·Trends in Endocrinology and Metabolism : TEM·Mugdha V JoglekarAnandwardhan A Hardikar
Aug 21, 2008·Clinical Genetics·A E Erson, E M Petty
Oct 28, 2008·Trends in Genetics : TIG·Inga ProkopenkoCecilia M Lindgren
Feb 6, 2010·Clinical Genetics·S BandieraA Henrion-Caude
May 25, 2010·Nature Reviews. Cancer·Bríd M RyanCurtis C Harris
Nov 26, 2010·Annals of the New York Academy of Sciences·Liana K Billings, Jose C Florez
Jul 27, 2011·Diabetes Care·Stefan S Fajans, Graeme I Bell
Mar 20, 2012·Cell·Frances M Ashcroft, Patrik Rorsman
Mar 20, 2012·Cell·Joshua T Mendell, Eric N Olson
Dec 12, 2012·Nature Medicine·Xiaogang WangYingxian Li
Jan 15, 2014·American Journal of Human Genetics·Qasim AyubYali Xue
Feb 11, 2014·Nature Genetics·UNKNOWN DIAbetes Genetics Replication And Meta-analysis (DIAGRAM) ConsortiumAndrew P Morris
Aug 2, 2014·Nucleic Acids Research·Chaochun LiuYe Ding
Aug 12, 2014·Biochemical and Biophysical Research Communications·Kazuo HaraTakashi Kadowaki

❮ Previous
Next ❯

Citations

Dec 9, 2016·Journal of Human Genetics·Naoko Hashimoto, Tomoaki Tanaka
Dec 10, 2016·Cell Biochemistry and Function·Jinggui HeShaobo Yang
Apr 7, 2017·Open Biology·Adrianna MoszyńskaRafał Bartoszewski
Mar 18, 2017·Scientific Reports·Karla Fabiana Brasil GomesMaria Elizabeth Rossi da Silva
May 31, 2019·In Vitro Cellular & Developmental Biology. Animal·Yamin LiuYajun Xie

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
Assay

Software Mentioned

Quanto
JMP

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.