Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio

Microbiology
Claudia MorenoRomilio T Espejo

Abstract

Analysis of the 16S rDNAs obtained from cultures of single colonies of either type collection strains or environmental strains of the genus Vibrio revealed the presence of polymorphism in every one of the strains examined. Polymorphism was detected by visualization of heteroduplexes produced after 16S rDNA PCR amplification, a procedure that allows for the screening of a large number of isolates. Amplified 16S rDNAs obtained from both Vibrio parahaemolyticus and an environmental strain were cloned. Their nucleotide sequences revealed differences of up to 2% among 16S rDNAs from the same strain. Polymorphic sites were concentrated in a recognized variable stem-loop of bacterial 16S rRNA that contained in some cases up to 83% of the total mismatches observed. Most of the substitutions present in the stem-loop region showed compensating base covariation. The accumulation of so many compensating changes in the stem-loop region implies that the divergence of the different versions of this stem-loop is relatively ancient. This divergence could be the result of either a selection process or a lateral transfer of independently evolved genes.

References

Jan 1, 1978·Journal of Bacteriology·H LamfromJ Abelson
Jan 1, 1992·International Journal of Systematic Bacteriology·M DorschE Stackebrandt
Aug 27, 1990·Biochimica Et Biophysica Acta·A SapagD E Draper
Jan 1, 1983·Methods in Enzymology·H C Birnboim
Dec 29, 1998·Journal of Bacteriology·K UedaM Kataoka
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·T AsaiC L Squires
Jun 5, 1999·The EMBO Journal·M I RechtJ D Puglisi
Oct 6, 1999·Current Opinion in Microbiology·J G Lawrence
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·K E Wommack, R R Colwell
Aug 5, 2000·Applied and Environmental Microbiology·I DahllöfS Kjelleberg
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

❮ Previous
Next ❯

Citations

Dec 18, 2014·Life·Laurie L RichardsonShawn R Campagna
Feb 3, 2016·International Journal of Biological Macromolecules·Yanbing ZhuHui Ni
Oct 2, 2012·Journal of Microbiological Methods·Rosalee S HellbergWilliam B Martin
Nov 13, 2014·World Journal of Microbiology & Biotechnology·Yanbing ZhuHuinong Cai
May 19, 2005·FEMS Microbiology Letters·Narjol González-EscalonaRomilio T Espejo
Dec 2, 2010·FEMS Microbiology Ecology·Sonja OberbeckmannGunnar Gerdts
Sep 29, 2009·FEMS Microbiology Ecology·Paola NavarreteJaime Romero
Nov 13, 2003·FEMS Microbiology Letters·Tom Coenye, Peter Vandamme
Nov 25, 2003·Journal of Applied Microbiology·H J OakeyA Thomas
Mar 5, 2011·Journal of Clinical Microbiology·María Soledad JiménezMarina Luquin
Sep 5, 2014·Journal of Clinical Microbiology·Lori M Gladney, Cheryl L Tarr
Jan 14, 2018·Extremophiles : Life Under Extreme Conditions·Yuliya V SamoylovaValentin N Parmon
Jun 18, 2010·Microbial Ecology·Silvana Teresa Tapia-PaniaguaMiguel Angel Moriñigo
Mar 7, 2019·International Journal of Systematic and Evolutionary Microbiology·Jian-Hang QuHai-Long Tian
Apr 13, 2007·Extremophiles : Life Under Extreme Conditions·Yasushi KageyamaKoki Horikoshi
Jun 26, 2017·Environmental Science and Pollution Research International·Qichun ZhangWarren A Dick
Nov 22, 2019·International Journal of Systematic and Evolutionary Microbiology·Jian-Hang QuLin-Feng Wang
Dec 9, 2003·Applied and Environmental Microbiology·S HaynesA E Douglas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.