Polymorphism in the tissue factor region is associated with basal but not endotoxin-induced tissue factor-mRNA levels in leukocytes

Journal of Thrombosis and Haemostasis : JTH
C MarsikB Jilma

Abstract

Tissue factor (TF) plays a central role during disseminated intravascular coagulation (DIC) in sepsis. We hypothesized that a frequent D/I polymorphism, at nucleotide position -1208 in the promoter region, could influence TF-mRNA and downstream coagulation. Basal- and lipopolysaccharide (LPS)-induced TF-mRNA expression, microparticle-associated TF-procoagulant activity and coagulation were determined in healthy men (n = 74) before and after endotoxin (LPS) infusion (2 ng kg(-1)). Basal values of TF-mRNA ranged between 34 and > 37.5 cycles. Baseline TF-mRNA levels significantly differed between genotypes: I/I carriers had almost 2-fold higher TF-mRNA levels compared to D/D carriers at baseline (P < 0.01). In accordance, higher levels of microparticle-associated TF-procoagulant activity could be seen in I/I carriers. However, the genotype did not affect basal or LPS-induced levels of prothrombin fragment F1+2, D-dimer or cytokines including tumor necrosis factor and interleukin-6. The TF-1208 polymorphism is functional in that it regulates basal TF-mRNA in circulating monocytes and circulating microparticle-associated TF-procoagulant activity in vivo, but does not influence the relative increase in TF-mRNA or coagulation activati...Continue Reading

References

Jan 6, 1999·Critical Care Medicine·G FriedmanJ L Vincent
Mar 2, 1999·American Journal of Respiratory and Critical Care Medicine·B JilmaO F Wagner
Oct 16, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·T PernerstorferW Speiser
Dec 12, 2001·Seminars in Thrombosis and Hemostasis·S Gando
Oct 19, 2002·Clinical Pharmacology and Therapeutics·Bernd JilmaHans Georg Eichler
Dec 17, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Georg EndlerRaute Sunder-Plassmann
May 20, 2003·British Journal of Haematology·Claudia MarsikBernd Jilma
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·P H ReitsmaJ C M Meijers
Feb 13, 2004·Thrombosis and Haemostasis·Carl-Erik Dempfle
Jun 12, 2004·Critical Care Medicine·Ulla DerhaschnigBernd Jilma
Mar 19, 2005·Critical Care : the Official Journal of the Critical Care Forum·Lucas T G J van EijkJohannes G van der Hoeven

❮ Previous
Next ❯

Citations

Feb 2, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Daniel P PotaczekAnetta Undas
Nov 30, 2007·Thrombosis Research·Waldemar WysokinskiRobert D McBane
Dec 6, 2006·Translational Research : the Journal of Laboratory and Clinical Medicine·Florian B Mayr, Bernd Jilma
Jul 6, 2011·Nature Reviews. Cardiology·Giuseppe LippiGiovanni Targher
Apr 26, 2006·Journal of Thrombosis and Haemostasis : JTH·M Levi
Jan 23, 2014·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Aerin KwonYoung Kyung Lee
Jul 9, 2009·Journal of Thrombosis and Haemostasis : JTH·M DE GaetanoL Iacoviello

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.