Polymorphism of F-actin assembly. 1. A quantitative phase diagram of F-actin

Biochemistry
A SuzukiT Ito

Abstract

We have made the first quantitative phase diagram of actin filament (F-actin) assembly represented by the concentration of F-actin and the chi parameter which characterizes solvent-solute interaction energy. We manipulated the chi value of F-actin by adding a high molecular weight poly- (ethylene glycol) with average molecular weight 6000 (PEG 6K). The preferential exclusion of PEG 6K from the region adjacent to F-actin increases the chi value of F-actin. We qualified the PEG 6K-induced increase of the chi value through analysis of the PEG-induced solubility change of protein. The phase diagram shows that F-actin changes its assembly structure from isotropic disordered distribution to anisotropic ordered phase of a lyotropic liquid crystalline with an increase in the concentration and to concentrated anisotropic ordered phase of a crystalline-like bundle with a small increase in chi respectively, in the physiological concentration range. The formation of the crystalline- like bundle suggests that some specific force may act between F-actin. The present results demonstrate that F-actin can take various assembly structures as observed in cytoplasm by itself, indicating that the versatility of F-actin assembly in cytoplasm may be ...Continue Reading

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Citations

Feb 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Itamar BorukhovAndrea J Liu
Nov 5, 1997·Clinical Obstetrics and Gynecology·D Dizon-Townson, K Ward
Mar 11, 1999·Obstetrical & Gynecological Survey·J D Hoffman, K Ward
Mar 26, 2011·Cytoskeleton·Florian Huber, Josef Käs
Jul 28, 2016·Polymers·Jörg SchnaußJosef A Käs
Apr 30, 2021·Soft Matter·Francis Cavanna, José Alvarado
Aug 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Kingo TakiguchiKenichi Yoshikawa

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