Polymorphism of mycotoxin biosynthetic genes among Fusarium equiseti isolates from Italy and Poland.

Journal of Applied Genetics
Lukasz StępieńJerzy Chełkowski

Abstract

Fusarium equiseti (Corda) Saccardo is a soil saprophyte and a weak pathogen, associated with several diseases of fruit and other crops in subtropical and tropical areas, but also in countries with temperate climate. A wide range of secondary metabolites has been identified among natural F. equiseti populations, with zearalenone (ZEA), fusarochromanone and fusarenon-X being the most common. In present study, the genetic diversity of strains from two populations (from Italy and Poland) was evaluated by analysing the translation elongation factor 1α (tef-1α) sequences, two polyketide synthases from the ZEA biosynthetic pathway (PKS13 and PKS4) and the TRI5 gene from the trichothecene biosynthetic pathway. ZEA was produced in rice cultures by 20 of the 27 tested isolates in concentrations ranging from 1.34 ng/g to 34,000 ng/g). The ability to produce enniatins and trichothecenes was evaluated in all strains by identifying esyn1, TRI13 and TRI4 genes. The presence of PKS4 and PKS13 genes was confirmed by polymerase chain reaction (PCR) in only some ZEA-producing isolates. Similarly, the TRI5 gene was found in 14 of the 27 isolates tested. This is likely to have been caused by the divergence of those genes between F. equiseti and F. ...Continue Reading

References

Jan 5, 2002·Applied and Environmental Microbiology·A LogriecoA Ritieni
Feb 15, 2003·FEMS Microbiology Letters·Prashant K MishraAlastair Culham
Mar 1, 2005·International Journal of Food Microbiology·Elzbieta Barbara KosiakMona Torp
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 23, 2008·International Journal of Food Microbiology·A MorettiA Logrieco
Nov 26, 2008·Journal of Applied Genetics·Łukasz StepieńJerzy Chełkowski
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·F BerthillerR Schuhmacher
Jan 11, 2011·Fungal Genetics and Biology : FG & B·Nancy J AlexanderRobert H Proctor
Feb 15, 2011·Fungal Biology·Lukasz StępieńAgnieszka Waśkiewicz
Jul 29, 2011·Journal of Applied Genetics·Lukasz StępieńAgnieszka Waśkiewicz

❮ Previous
Next ❯

Citations

Apr 11, 2013·Journal of Applied Genetics·Łukasz StępieńAgnieszka Waśkiewicz
Dec 3, 2014·International Journal of Food Microbiology·Łukasz StępieńKarolina Wilman

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence

Software Mentioned

MEGABLAST
MEGA4
ClustalW
BLASTn

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.