Polymorphism of the polyketide synthase gene phID in biocontrol fluorescent pseudomonads producing 2,4-diacetylphloroglucinol and comparison of PhID with plant polyketide synthases

Molecular Plant-microbe Interactions : MPMI
Alban RametteG Défago

Abstract

Many biocontrol fluorescent pseudomonads can protect plants from soilborne fungal pathogens through production of the antifungal secondary metabolite 2,4-diacetylphloroglucinol (Phl). One of the phl biosynthetic genes, phlD, encodes a polyketide synthase similar to plant chalcone synthases. Here, restriction analysis of phlD from 39 Phl+ biocontrol fluorescent pseudomonads yielded seven different banding patterns. The gene was sequenced in seven strains, representing the different restriction patterns. Cluster analysis of phlD restriction data or phlD sequences indicated that phlD polymorphism was high, and two main clusters were obtained when predicted PhlD sequences were compared. When the seven PhlD sequences were studied with those of other procaryotic polyketide synthases (gram-positive bacteria) and plant chalcone synthases, however, Phl+ pseudomonads, gram-positive bacteria, and plants clustered separately. Yet, sequence analysis of active site regions for PhlD and plant chalcone synthases revealed that PhlD can be considered a member of the chalcone synthase family, which may be interpreted as convergent evolution of key enzymes involved in secondary metabolism. For the 39 Phl+ pseudomonads, a relationship was found amo...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Nei, W H Li
Oct 1, 1991·Applied and Environmental Microbiology·M N VincentE A Pierson
Jun 1, 1986·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M Staemmler, K Gersonde
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1993·International Review of Cytology·C R Martin
Jan 1, 1996·International Journal of Systematic Bacteriology·P NormandA K Misra
Sep 7, 1999·Nature·N FunaS Horinouchi
May 2, 2000·Applied and Environmental Microbiology·B B McSpadden GardenerD M Weller
Aug 12, 2000·International Journal of Systematic and Evolutionary Microbiology·Y AnzaiH Oyaizu
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J CookD S Kim
Mar 10, 2004·Applied and Environmental Microbiology·Brion DuffyGeneviève Défago
Mar 1, 1997·Applied and Environmental Microbiology·J M RaaijmakersL S Thomashow

❮ Previous
Next ❯

Citations

Jul 25, 2007·Die Naturwissenschaften·Christine Picard, Marco Bosco
Nov 22, 2011·Applied Microbiology and Biotechnology·Fang Yang, Yujin Cao
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·S Heeb, D Haas
Oct 24, 2008·Phytopathology·Brian B McSpadden GardenerElizabeth Lutton
Oct 24, 2008·Phytopathology·Brian B McSpadden Gardener
Feb 3, 2009·Applied and Environmental Microbiology·Jennifer A MoynihanE Fidelma Boyd
Mar 10, 2004·Applied and Environmental Microbiology·Brion DuffyGeneviève Défago
Jul 31, 2002·Annual Review of Phytopathology·David M WellerLinda S Thomashow
Aug 8, 2009·International Journal of Systematic and Evolutionary Microbiology·Eun Ju ChoiHyun Ok Yang
Jun 1, 2004·FEMS Microbiology Ecology·Matthias P LutzBrion Duffy
Mar 22, 2006·Microbiology·Josselin Bodilis, Sylvie Barray

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.