Polymorphisms in major histocompatibility complex genes and its associations with milk quality in Murrah buffaloes

Tropical Animal Health and Production
Ana C FreitasH Tonhati

Abstract

Animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. Genes of the major histocompatibility complex (MHC) are important molecular markers for disease resistance that could be applied for genetic selection. The aim of this study was to identify single nucleotide polymorphisms (SNPs) and haplotypes in DRB2, DRB3, DMA, and DMB genes in Murrah breed and to analyze the association between molecular markers and milk, fat, protein and mozzarella production, fat and protein percentage, and somatic cell count. Two hundred DNA samples from Murrah buffaloes were used. The target regions of candidate genes were amplified by polymerase chain reaction (PCR) followed by sequencing and identification of polymorphisms. Allele and genotype frequencies, as well as linkage disequilibrium between SNPs, were calculated. Genotypes were used in association analyses with milk production and quality traits. Except for the DMA gene, identified as monomorphic, the other genes presented several polymorphisms. The DMB, DRB2, and DRB3 genes presented two, six, and seven SNPs, respectively. Fifty-sev...Continue Reading

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Citations

Apr 24, 2021·Tropical Animal Health and Production·Mohammed Hamed EldawyMohammed Hamdy Farouk

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

OligoAnalyzer
CodonCode Aligner
Image Analysis
STAT
Primer3Plus
Plink
Haploview
SAS

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