Polymorphisms in the coding region of mtDNA and effects on clinical outcome of unrelated bone marrow transplantation

Bone Marrow Transplantation
Y IshikawaT Juji

Abstract

The entire protein-coding region was divided into 45 fragments, separately amplified and analyzed for polymorphism by the PCR-SSCP (single-strand conformation polymorphism) method. The effect of polymorphism mismatching on the clinical outcome of unrelated bone marrow transplantation was studied to clarify whether products from mtDNA become minor antigens. Variability in PCR-SSCP pattern combinations of the 45 fragments suggests that each individual has a different polymorphism combination in the protein-coding region if all the coding regions were compared at the nucleotide sequence level. Nonsynonymous polymorphisms were found at relatively high frequency in MTATP8 and MTND3. Both the polymorphisms with and without substitution matched the peptide-binding motifs of HLA-A*0201. The effects of the polymorphism matching were retrospectively analyzed in 340 recipients transplanted with HLA-A, -B, -DRB1 allele-matched bone marrow from unrelated donors. There were no effects of polymorphism matching on the incidence of acute GVHD and cumulative disease-free survival. These results suggest that polymorphisms which generate peptides, with and without substitutions, that bind the same HLA molecule hardly influence GVHD because the dif...Continue Reading

References

Apr 1, 1991·The Journal of Experimental Medicine·J D DaviesD B Wilson
Jan 1, 1996·Immunogenetics·V M Dabhi, K F Lindahl

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Citations

Dec 17, 2009·Digestive Diseases and Sciences·Masatoshi YanagisawaMasatomo Mori
Oct 10, 2013·Mitochondrial DNA·Gen-Hong MaoShi-Bo Tao
Oct 11, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Julie A RossMichael R Verneris
May 15, 2007·Tissue Antigens·B AfzaliM P Hernandez-Fuentes
Apr 30, 2002·Bone Marrow Transplantation·Y IshikawaT Juji

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