PMID: 2507552Oct 1, 1989Paper

Polymorphonuclear leukocyte adherence induces actin polymerization by a transduction pathway which differs from that used by chemoattractants

The Journal of Cell Biology
F S SouthwickS H Zigmond

Abstract

Nitrobenzoxadiazole-phallacidin in combination with quantitative fluorescent microscopy have been used to measure F-actin concentrations in human polymorphonuclear leukocytes (PMN) as they adhere to a plastic surface. Like stimulation with chemoattractants, adherence is associated with a twofold rise in F-actin content. However unlike the rapid rise in F-actin induced by chemoattractants which peaks within 30 s, actin assembly induced by adherence is slower, maximum F-actin values not being observed until 10 min. Furthermore the rise in F-actin induced by adherence is persistent, remaining constant over 60 min while F-actin returns to near basal levels after 20 min exposure to chemoattractant. The combination of adherence (5 min) followed by chemoattractant (FMLP 5 x 10(-8) M for 40 s) resulted in an additive rise in F-actin content to greater than threefold over unstimulated values. Unlike chemoattractant induced actin assembly, adherence-associated PMN actin polymerization was not inhibited by pertussis toxin, but was markedly reduced by lowering extracellular Ca2+. Fluorescent micrographs of adherent PMN stained with nitrobenzoxadiazole-phallacidin revealed F-actin in the lamellipodia and in small foci on the adherent surfac...Continue Reading

References

Jan 1, 1985·Annual Review of Cell Biology·T P StosselH L Yin
Oct 1, 1987·The Journal of Cell Biology·J A Cooper
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B A KruskalF R Maxfield
Aug 1, 1986·The Journal of Cell Biology·G W Conrad, T J Rink
Sep 1, 1984·The Journal of Cell Biology·P J WallaceM A Lichtman
Nov 1, 1983·Analytical Biochemistry·C S Heacock, J R Bamburg
Jun 1, 1980·The Journal of Cell Biology·S J Sullivan, S H Zigmond

❮ Previous
Next ❯

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·D V ZhelevR M Hochmuth
Dec 15, 1996·Journal of Cellular Biochemistry·M OkuyamaM Monden
Feb 1, 1996·Journal of Cellular Physiology·C J CornishC L Geczy
Jan 1, 1993·Cell Motility and the Cytoskeleton·J S WangK S Zaner
Jan 1, 1994·Cell Motility and the Cytoskeleton·M Y Niu, V T Nachmias
May 8, 1998·Cell Biology International·M Keresztes, Z Lajtos
Jul 1, 1995·Archives of Environmental Contamination and Toxicology·M Macia, M Hernández
Jan 5, 2000·Journal of Immunological Methods·M Torres, T D Coates
Mar 17, 2009·Nature Cell Biology·J Bradley ZucheroR Dyche Mullins
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J RichterT Andersson
Jun 1, 1990·The Journal of Cell Biology·G P DowneyS Grinstein
Aug 1, 1991·The Journal of Cell Biology·M H Symons, T J Mitchison
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Jan 1, 1997·Molecular Biology of the Cell·J S WangK S Zaner
Dec 10, 1999·The Plant Cell·B C GibbonC J Staiger
Apr 8, 2009·Infection and Immunity·Sarah E SzarowiczFrederick S Southwick
May 19, 1998·Journal of Endocrinological Investigation·G ZoppiniM Muggeo
Jul 29, 1998·The Journal of Cell Biology·E A ClarkR O Hynes
Apr 1, 1994·Cytometry·K RadosevićJ Greve
Oct 11, 1996·Biochimica Et Biophysica Acta·C Geczy
Feb 1, 1991·Current Opinion in Cell Biology·E J Luna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.