Polymorphonuclear leukocyte-myocyte interaction: an early event in collar-induced rabbit carotid intimal thickening

Experimental Cell Research
Elena DonettiAlberto Corsini

Abstract

The importance of mononuclear leukocyte (MO) adhesion to dysfunctional endothelium and migration to the subendothelial space in the early phases of atherogenesis is well established. Few studies have addressed the relevance of polymorphonuclear leukocytes (PMNs) in the context of evolving lesions, and nothing is known about PMN interaction with vascular smooth muscle cells (SMCs). In this study, we investigated leukocyte/SMC interactions in a model of rabbit carotid injury induced by placement of a silastic collar. This procedure leads to the development of intimal thickening characterized by SMC accumulation preceded by an abundant leukocyte infiltration. By transmission electron microscopy and immunocytochemistry, we demonstrated the occurrence of PMN infiltration starting at 6 h and ceasing within 72 h after collar placement. A previously unknown extensive interaction between medial myocytes and PMNs was detected, referring to emperipolesis, an active phenomenon of cells engulfing other cells in a process other than phagocytosis. PMNs, but not MOs, were internalized by SMCs, which showed ultrastructural features intermediate between the true contractile and the fully synthetic phenotype without exhibiting any sign of injury....Continue Reading

Citations

Nov 5, 2005·European Journal of Clinical Investigation·S BhardwajS Ylä-Herttuala
Oct 18, 2005·The British Journal of Dermatology·E DonettiN Gagliano
Oct 17, 2013·Cardiovascular Research·Hamish C G ProsserChristina A Bursill
May 31, 2008·Expert Review of Cardiovascular Therapy·Shalini BhardwajSeppo Ylä-Herttuala
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Roberta BaettaAlberto Corsini
Apr 25, 2008·Current Protocols in Immunology·Alice BeareIan Nicholson
Aug 13, 2003·Journal of Thrombosis and Haemostasis : JTH·C DavisI J Sarembock
Feb 19, 2013·Journal of Cardiac Surgery·Xiao-Wen WangXiao-Yong Xiang
Jan 25, 2019·ACS Applied Materials & Interfaces·Bingcheng YiYanzhong Zhang

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