Polymorphonuclear leukocyte phagocytic function increases in plasminogen knockout mice

Thrombosis Research
Paul K WittingD B Brieger

Abstract

Mice lacking plasminogen (PG-/-) require alternative pathways of fibrinolysis for survival. This may depend on polymorphonuclear leukocytes (PMN) that can clear soluble and insoluble fibrin(ogen) through PG-independent processes. Our objective was to demonstrate that PMNs from PG-/- mice exhibit increased Mac-1 dependent phagocytic activity, which may explain their increased fibrin(ogen)lytic activity compared with wild type (PG+/+) mice. Phagocytic activity of PMNs from PG-/- and PG+/+ mice was compared following exposure to Staphylococcus aureus (S. aureus) particles and the expression of Mac-1 was assessed in parallel by flow cytometric analysis. Resistance to phorbol-12-myristate-13-acetate (PMA)-induced cell death was compared between PMNs from the different genotypes. Stimulation of PG-/- PMNs by opsonized S. aureus diluted in PG-/- plasma significantly increased phagocytosis (15-fold) compared with stimulation of PG+/+ PMNs in PG+/+ plasma. Incubation of PG-/- PMNs with PG+/+ plasma (control) or PG-/- plasma supplemented with human PG inhibited this increased phagocytic activity. Mac-1 cell surface density increased 6.2+/-1.0-fold in PG-/- PMNs versus 2.9+/-0.6-fold in PG+/+ PMNs (P < 0.01) indicating that Mac-1 may be a...Continue Reading

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Citations

Jan 13, 2015·Transfusion Medicine Reviews·Dominik F Draxler, Robert L Medcalf
Jan 15, 2014·Infection and Immunity·Alanna M SpeesAndreas J Bäumler
Sep 20, 2017·Journal of Thrombosis and Haemostasis : JTH·R L Medcalf
Jul 16, 2008·Free Radical Biology & Medicine·Andrea J Szuchman-SapirPaul K Witting

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