PMID: 9549629Apr 29, 1998Paper

Polymyxin B inhibits biphasic calcium phosphate degradation induced by lipopolysaccharide-activated human monocytes/macrophages

Journal of Biomedical Materials Research
S KimakheG Daculsi

Abstract

Numerous cell types, such as monocytes and osteoclasts, are involved in calcified matrix degradation. In this context, calcium-phosphate ceramics present similar degradation processes in vivo and in vitro to those found in a natural calcified substrate. As the monocyte/macrophage lineage is among the first cells to appear in ceramic implantation sites, it is a key protagonist in inflammatory reaction and biodegradation mechanisms. This study investigated the ability of human monocytes/macrophages activated by various agents [lipopolysaccharides (LPS), polymyxin B (PMB)] to degrade biphasic calcium-phosphate ceramics. PMB sulfate is a bacteriostatic antibiotic that modulates LPS-induced cell activities in vivo and in vitro. Degradation pits (about 10 microns) produced on the pellet surface by these monocytes were discrete, with well defined margins. LPS increased the degradation of calcium-phosphate ceramic (number of lacunae, mean pellet surface area degraded) in a dose-dependent manner whereas polymyxin B downmodulated it significantly. The addition of 2 micrograms/mL of polymyxin B reduced the number of degradation lacunae and the extent of degraded surface area induced by 0.1 microgram/mL LPS by 87% and 64%, respectively. Th...Continue Reading

References

Jul 1, 1992·The Journal of Bone and Joint Surgery. British Volume·Y ShintoK Ono
May 1, 1991·Journal of Periodontal Research·G R Mundy
Aug 1, 1991·Journal of Periodontology·P StashenkoS S Socransky
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·C SaginarioA Vignery
Sep 1, 1996·Endocrinology·Y FujikawaN A Athanasou
Oct 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M D BenahmedG Pradal
Jan 1, 1997·Journal of Biomedical Materials Research·M BenahmedG Daculsi
Feb 1, 1997·Journal of Biomedical Materials Research·J GuicheuxG Daculsi
Dec 9, 1992·Clinical Materials·R Z LeGeros

❮ Previous
Next ❯

Citations

Apr 18, 2013·American Journal of Respiratory Cell and Molecular Biology·Kiflai BeinGeorge D Leikauf
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Hwa LeeHaibo Zhang
Jul 18, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jeanne-Marie DamerauBernd Stadlinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.