PMID: 3755358Jun 3, 1986Paper

Poly(N-acetyllactosaminyl) oligosaccharides in glycoproteins of PC12 pheochromocytoma cells and sympathetic neurons

Biochemistry
R U MargolisR U Margolis

Abstract

Endo-beta-galactosidase treatment of glycopeptides derived from the trypsinate and membranes of PC12 pheochromocytoma cells and cultured sympathetic neurons demonstrated the presence of poly(N-acetyllactosaminyl) units on tri- and tetraantennary oligosaccharides, some of which have a core fucose residue and a 2,6-substituted alpha-linked mannose residue. Nerve growth factor induced differentiation of the PC12 cells led to a small but significant decrease in the proportion of these oligosaccharides. Poly(N-acetyllactosaminyl) oligosaccharides were also identified in a major 230 000-Da cell-surface glycoprotein (the nerve growth factor inducible large external, or NILE, glycoprotein) of PC12 cells and appear to account for much or all of the difference in size between this glycoprotein as compared to the immunochemically cross-reactive 205 000-Da species present in postnatal brain. Glycoproteins containing poly(N-acetyllactosaminyl) oligosaccharides were selectively labeled by treatment of PC12 cells with endo-beta-galactosidase to expose N-acetylglucosamine residues, followed by incubation with galactosyltransferase and UDP-[14C]galactose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography revealed the pr...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T Krusius, E Ruoslahti
Feb 1, 1986·The Journal of Cell Biology·D R FriedlanderG M Edelman
Jul 1, 1980·Experimental Cell Research·L G RodaR A Hogue-Angeletti
Feb 1, 1984·Archives of Biochemistry and Biophysics·R K MargolisR U Margolis
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M FukudaS Hakomori

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Citations

Dec 1, 1987·Journal of Neurochemistry·R U MargolisR K Margolis
Dec 1, 1988·Journal of Neurochemistry·R U MargolisR K Margolis
Jul 16, 2013·The Journal of Craniofacial Surgery·Jin LanYongming Qiu
Feb 28, 1989·Biochemical and Biophysical Research Communications·M L Rasilo, T Yamagata

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