Polynucleotide: adenosine glycosidase activity of immunotoxins containing ribosome-inactivating proteins

Journal of Drug Targeting
Luigi BarbieriFiorenzo Stirpe

Abstract

Polynucleotide:adenosine glycosidases (rRNA N-glycosidases, EC 3.2.2.22, more commonly known as ribosome-inactivating proteins, RIP) are a numerous family of plant and bacterial enzymes, shown to release also adenine from DNA in vitro. They are well suited for the preparation of specifically toxic conjugates with several carriers, including monoclonal antibodies (immunotoxins). Here we show that (i) immunotoxins containing various PNAG (dianthin, gelonin, momordin I, PAP-S, PDS-2, ricin A-chain, saporin-L1, saporin-S6) all act on DNA; (ii) activity on DNA in vitro is less compromised by disulphide linkage to antibody than is inhibition of cell-free protein translation; and (iii) specific cytotoxicity of immunotoxin does not correlate with substrate specificity.

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Lee-HuangH C Chen
Aug 1, 1990·AIDS Research and Human Retroviruses·M S McGrathI Gaston
Jul 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M G BattelliF Stirpe
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M S McGrathJ Marsh
Mar 1, 1983·Antimicrobial Agents and Chemotherapy·G J TeltowG M Aron
Dec 15, 1994·Nature·L BarbieriF Stirpe
Feb 1, 1995·Cancer Immunology, Immunotherapy : CII·A BolognesiF Stirpe
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Nov 4, 1996·International Journal of Cancer. Journal International Du Cancer·A BolognesiF Stirpe

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Citations

Nov 10, 2011·Toxins·Assaf Shapira, Itai Benhar
Feb 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Letizia PolitoAndrea Bolognesi
Feb 22, 2005·Protein Expression and Purification·Ramarao VepacheduJorge M Vivanco
Jun 25, 2016·Toxins·Massimo BortolottiLetizia Polito
Jan 31, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea BolognesiLetizia Polito
May 29, 2007·Oncogene·I Zafir-LavieY Reiter
Jun 9, 2019·Toxins·Letizia PolitoAndrea Bolognesi
Jul 27, 2002·Journal of Immunotherapy·Cinzia RicciPier-Luigi Lollini
May 26, 2017·Biomedicines·Letizia PolitoMassimo Bortolotti
Feb 22, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hossein AllahyariJafar Amani
Mar 13, 2002·Archives of Biochemistry and Biophysics·Wen-Jun HeFiorenzo Stirpe
Mar 16, 2005·Biochemistry·Tim K AmukeleVern L Schramm

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