Polyol additives modulate the in vitro stability and activity of recombinant human phenylalanine hydroxylase

Applied Biochemistry and Biotechnology
Cátia NascimentoPaula Leandro

Abstract

Phenylketonuria (PKU; OMIM 261600), the most common disorder of amino acid metabolism, is caused by a deficient activity of human phenylalanine hydroxylase (hPAH). Although the dietetic treatment has proven to be effective in preventing the psycho-motor impairment, much effort has been made to develop new therapeutic approaches. Enzyme replacement therapy with hPAH could be regarded as a potential form of PKU treatment if the reported in vitro hPAH instability could be overcome. In this study, we investigated the effect of different polyol compounds, e.g. glycerol, mannitol and PEG-6000 on the in vitro stability of purified hPAH produced in a heterologous prokaryotic expression system. The recombinant human enzyme was stored in the presence of the studied stabilizing agents at different temperatures (4 and -20 degrees C) during a 1-month period. Protein content, degradation products, specific activity, oligomeric profile and conformational characteristics were assessed during storage. The obtained results showed that the use of 50% glycerol or 10% mannitol, at -20 degrees C, protected the enzyme from loss of its enzymatic activity. The determined DeltaG(0) and quenching parameters indicate the occurrence of conformational chang...Continue Reading

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Citations

Oct 11, 2011·Neurochemical Research·Jonathan E FernsNorbert W Seidler
Apr 8, 2020·Molecular Therapy. Methods & Clinical Development·Seemin S AhmedOmar L Francone
Aug 8, 2021·International Journal of Molecular Sciences·Paulo R LinoAntónio J Almeida

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