Polyol pathway and NADPH-dependent reductases in dog leukocytes

Journal of Diabetes and Its Complications
Shigeru FukaseP F Kador

Abstract

Recent reports suggest that excess amounts of sugar alcohol are linked to leukocyte dysfunctions associated with diabetes. As the polyol pathway has not been firmly established in leukocytes, we have investigated NADPH-dependent reductases and sugar alcohol formation in dog leukocytes. NADPH-dependent reductase activity was observed with DL-glyceraldehyde as substrate in both mononuclear and polymorphonuclear leukocytes isolated from dog. By chromatofocusing, this activity corresponded primarily to aldehyde reductase rather than aldose reductase. The enzymatic conversion of glucose to the sugar alcohol sorbitol in leukocytes was confirmed in vitro by 19F nuclear magnetic resonance (NMR) spectroscopy using 3-deoxy-3-fluoro-D-glucose as substrate. The NMR spectrum obtained after incubation with 10 Mm 3-deoxy-3-fluoro-D-glucose at 37 degrees C for 24 h displayed newly formed 3-deoxy-3-fluoro-D-sorbitol and 3-deoxy-3-fluoro-D-fructose peaks with both mononuclear and polymorphonuclear leukocytes. Sugar alcohol production in leukocytes from galactose-fed dogs was also observed in vivo. Galactitol accumulation was consistently observed by gas chromatography to occur in mononuclear cells while only trace amounts of galactitol were obse...Continue Reading

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Citations

Mar 18, 2000·European Journal of Pharmacology·J W CruzZ B Fortes
Feb 8, 2013·BMC Veterinary Research·Anelise M BoscoPaulo C Ciarlini
Dec 30, 2010·Journal of Periodontology·Peter F KadorRichard A Reinhardt
Aug 10, 2011·Journal of Cellular Biochemistry·Na LiHengyi Xiao
Feb 27, 2003·Chemico-biological Interactions·Sanai SatoMartin J Lizak

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