Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling

Frontiers in Plant Science
María Francisca AguayoMichael Handford

Abstract

Polyols are enzymatically-produced plant compounds which can act as compatible solutes during periods of abiotic stress. Nicotinamide adenine dinucleotide(+)-dependent SORBITOL DEHYDROGENASE (SDH, E. C. 1.1.1.14) from Arabidopsis thaliana L. sorbitol dehydrogenase (AtSDH) is capable of oxidizing several polyols including sorbitol, ribitol, and xylitol. In the present study, enzymatic assays using recombinant AtSDH demonstrated a higher specificity constant for xylitol compared to sorbitol and ribitol, all of which are C2 (S) and C4 (R) polyols. Enzyme activity was reduced by preincubation with ethylenediaminetetraacetic acid, indicating a requirement for zinc ions. In humans, it has been proposed that sorbitol becomes part of a pentahedric coordination sphere of the catalytic zinc during the reaction mechanism. In order to determine the validity of this pentahedric coordination model in a plant SDH, homology modeling, and Molecular Dynamics simulations of AtSDH ternary complexes with the three polyols were performed using crystal structures of human and Bemisia argentifolii (Genn.) (Hemiptera: Aleyrodidae) SDHs as scaffolds. The results indicate that the differences in interaction with structural water molecules correlate very ...Continue Reading

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Citations

Oct 26, 2020·Functional Plant Biology : FPB·Afaf AlmaghamsiDouglas D Archbold
Feb 7, 2019·Biochemistry·Matías D HartmanCarlos M Figueroa

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Methods Mentioned

BETA
enzymatic titration
gel filtration

Software Mentioned

NAMD
Verify3D
AutoDock Tools
GalaxyLoop
Verify
GalaxyWeb
SigmaPlot
Autodock Vina
MultiSeq
BLAST

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