Abstract
Wild-type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle-T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle-T-transformed mouse bEND cells prepared from PECAM-1-/- mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM-1+/+ bEND cells. PECAM-1-/- bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM-1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM-1+/+ bEND cells. Therefore, lack of PECAM-1 expression impacts polyoma middle-T-transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary-like structures in Matrigel.
References
Apr 1, 1990·The Journal of Cell Biology·S M AlbeldaC A Buck
Aug 10, 1990·Cell·R MontesanoL Orci
Nov 20, 1987·Cell·V L BautchD Hanahan
Jun 16, 1989·Cell·R L WilliamsE F Wagner
Jan 15, 1988·Cell·R L WilliamsE F Wagner
Jul 1, 1995·The Journal of Cell Biology·L PialiB A Imhof
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Sheibani, W A Frazier
Apr 1, 1995·Journal of Leukocyte Biology·W A Muller
Aug 1, 1993·The Journal of Experimental Medicine·W A MullerD M Phillips
Aug 2, 1996·The Journal of Biological Chemistry·J SunH M DeLisser
Jul 1, 1997·Molecular Biology of the Cell·N SheibaniW A Frazier
May 16, 1998·Molecular Biology of the Cell·N Sheibani, W A Frazier
Jan 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·N SheibaniW A Frazier
Sep 12, 2000·Molecular Biology of the Cell·C W WongB A Imhof
Mar 10, 2001·Blood·R D ThompsonS Nourshargh
Dec 6, 2001·The American Journal of Pathology·Y YuJ Bischoff
Feb 22, 2002·Medicinal Research Reviews·Helena Yusuf-MakagiansarTeruna J Siahaan
Nov 6, 2002·The Journal of Experimental Medicine·John DangerfieldSussan Nourshargh
Dec 7, 2002·Blood·Christopher D O'BrienSteven M Albelda
Feb 25, 2003·The American Journal of Pathology·Anna SolowiejJoseph A Madri
Mar 21, 2003·Biochemical and Biophysical Research Communications·Purba BiswasJoseph A Madri
Mar 22, 2003·Blood·Cunji GaoPeter J Newman
Apr 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter J Newman, Debra K Newman
Aug 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dita GratzingerJoseph A Madri
Aug 26, 2003·Journal of Cellular Biochemistry·Jianqiang Wu, Nader Sheibani
Oct 2, 2003·Current Opinion in Cell Biology·Neta Ilan, Joseph A Madri
Citations
Dec 11, 2008·Transgenic Research·Qin XuZhiyuan Zhang
Nov 25, 2005·Leukemia·B YatsulaA S Perkins
Jul 14, 2005·Current Opinion in Pediatrics·Francine Blei
Sep 3, 2010·American Journal of Physiology. Cell Physiology·SunYoung ParkNader Sheibani
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Qiong Huang, Nader Sheibani
Feb 6, 2009·American Journal of Physiology. Cell Physiology·Elizabeth A ScheefNader Sheibani
Jul 27, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cathy GrutzmacherChristine M Sorenson
Oct 19, 2012·American Journal of Physiology. Renal Physiology·Cathy GrutzmacherChristine M Sorenson
Dec 16, 2011·American Journal of Physiology. Renal Physiology·Nader SheibaniChristine M Sorenson
Apr 8, 2006·Blood·Mandana MansouriKlaus Früh
Dec 17, 2011·PLoS Pathogens·Terri A DiMaioMichael Lagunoff
Jan 27, 2009·The Journal of Craniofacial Surgery·June K Wu, Jan K Kitajewski
Nov 22, 2007·Microvascular Research·Terri A DiMaio, Nader Sheibani
Mar 22, 2008·The FEBS Journal·Takashi YamaguchiHirotake Ichise
Jul 4, 2006·The American Journal of Pathology·Purba BiswasJoseph A Madri
May 16, 2015·Clinical Science·Sunyoung ParkNader Sheibani
Oct 1, 2014·Molecular and Cellular Biology·Masayuki Tsuneki, Joseph A Madri
Nov 30, 2006·Journal of Cellular Physiology·Nader SheibaniChristine M Sorenson
Jan 28, 2006·Journal of Cellular Biochemistry·Yongji Wang, Nader Sheibani
Jun 15, 2007·American Journal of Physiology. Cell Physiology·Shuji KondoChristine M Sorenson
Apr 18, 2008·American Journal of Physiology. Cell Physiology·Shuji KondoChristine M Sorenson
Sep 19, 2018·BMC Ophthalmology·Xinyi DingHaixiang Wu
Jun 5, 2020·Cell Host & Microbe·Julia BruggisserHorst Posthaus