Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice

Journal of Cellular Physiology
Terri A RothermelNader Sheibani

Abstract

Wild-type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle-T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle-T-transformed mouse bEND cells prepared from PECAM-1-/- mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM-1+/+ bEND cells. PECAM-1-/- bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM-1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM-1+/+ bEND cells. Therefore, lack of PECAM-1 expression impacts polyoma middle-T-transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary-like structures in Matrigel.

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Citations

Jul 14, 2005·Current Opinion in Pediatrics·Francine Blei
Sep 3, 2010·American Journal of Physiology. Cell Physiology·SunYoung ParkNader Sheibani
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Qiong Huang, Nader Sheibani
Feb 6, 2009·American Journal of Physiology. Cell Physiology·Elizabeth A ScheefNader Sheibani
Jul 27, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cathy GrutzmacherChristine M Sorenson
Oct 19, 2012·American Journal of Physiology. Renal Physiology·Cathy GrutzmacherChristine M Sorenson
Dec 16, 2011·American Journal of Physiology. Renal Physiology·Nader SheibaniChristine M Sorenson
Jan 27, 2009·The Journal of Craniofacial Surgery·June K Wu, Jan K Kitajewski
Nov 22, 2007·Microvascular Research·Terri A DiMaio, Nader Sheibani
Jul 4, 2006·The American Journal of Pathology·Purba BiswasJoseph A Madri
May 16, 2015·Clinical Science·Sunyoung ParkNader Sheibani
Oct 1, 2014·Molecular and Cellular Biology·Masayuki Tsuneki, Joseph A Madri
Nov 30, 2006·Journal of Cellular Physiology·Nader SheibaniChristine M Sorenson
Jun 15, 2007·American Journal of Physiology. Cell Physiology·Shuji KondoChristine M Sorenson
Apr 18, 2008·American Journal of Physiology. Cell Physiology·Shuji KondoChristine M Sorenson

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