Polyomavirus large T can support DNA replication in human cells

Virology
F SverdrupE J Androphy

Abstract

Human cells are generally thought to be nonpermissive for polyomavirus (Py) DNA replication. Using transient transfection, we show that Py large T-antigen (LT) was able to support replication of a Py origin-containing plasmid in two human cell lines. Replication supported by LT in human cells was specific for the Py origin and required its enhancer sequences, as well as the previously reported critical phosphorylation sites within LT. Py replication efficiency was comparable to that of papillomavirus E1 and E2 activated DNA replication in transient assays performed in human 293 and C-33A cells. Previous analysis of DNA replication in vitro has pointed to polymerase alpha-primase as a specificity determinant for polyomavirus. The data presented here imply that in certain cellular environments, Py LT must functionally interact with human polymerase alpha-primase to permit DNA replication.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M ChiangL T Chow
Jul 1, 1991·Journal of Virology·P F Lambert
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Jan 1, 1989·Annual Review of Biochemistry·M D Challberg, T J Kelly
Dec 1, 1989·Journal of Virology·E R BennettJ A Hassell
May 1, 1988·Journal of Virology·C R MuellerJ A Hassell
Dec 15, 1987·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C R WobbeJ Hurwitz
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y MurakamiJ Hurwitz
Apr 1, 1987·Journal of Virology·B J Bockus, B Schaffhausen
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Mar 1, 1974·Virology·R GruenM Fogel
Nov 15, 1984·Nature·J de VilliersR Kamen
Jun 1, 1980·Virology·I DvoretzkyD R Lowy
Sep 19, 1980·Science·K W PedenD Nathans
Dec 20, 1982·Nucleic Acids Research·J de VilliersW Schaffner
Sep 1, 1983·Journal of Virology·W J MullerJ A Hassell
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·V Gopalakrishnan, S A Khan
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·O V GjørupB S Schaffhausen
Jun 15, 1994·European Journal of Biochemistry·F StadlbauerH P Nasheuer
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z Liu, G G Carmichael

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