Polyomavirus small t antigen prevents retinoic acid-induced retinoblastoma protein hypophosphorylation and redirects retinoic acid-induced G0 arrest and differentiation to apoptosis

Journal of Virology
Andrew YenSusi Varvayanis

Abstract

Polyomavirus small t antigen (ST) impedes late features of retinoic acid (RA)-induced HL-60 myeloid differentiation as well as growth arrest, causing apoptosis instead. HL-60 cells were stably transfected with ST. ST slowed the cell cycle, retarding G2/M in particular. Treated with RA, the ST transfectants continued to proliferate and underwent apoptosis. ST also impeded the normally RA-induced hypophosphorylation of the retinoblastoma tumor suppressor protein consistent with failure of the cells to arrest growth. The RA-treated transfectants expressed CD11b, an early cell surface differentiation marker, but inducible oxidative metabolism, a later and more mature functional differentiation marker, was largely inhibited. Instead, the cells underwent apoptosis. ST affected significant known components of RA signaling that result in G0 growth arrest and differentiation in wild-type HL-60. ST increased the basal amount of activated ERK2, which normally increases when wild-type cells are treated with RA. ST caused increased RARalpha expression, which is normally down regulated in RA-treated wild-type cells. The effects of ST on RA-induced myeloid differentiation did not extend to monocytic differentiation and G0 arrest induced by 1,...Continue Reading

References

Oct 1, 1978·Experimental Cell Research·A Yen, A B Pardee
Jun 1, 1990·Molecular and Cellular Biology·B CohenB S Schaffhausen
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Aug 1, 1983·Molecular and Cellular Biology·C AsselinM Bastin
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
May 1, 1995·Journal of Neurochemistry·H M RoderW Schröder
May 26, 1995·The Journal of Biological Chemistry·W SuT M Roberts
May 1, 1995·Archives of Microbiology·G T Marczynski, L Shapiro
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·P J LewisJ Errington
Nov 1, 1993·Journal of Cellular Physiology·A YenJ D Platko
Feb 1, 1994·Journal of Virology·P S HolmanB S Schaffhausen
Nov 20, 1998·Molecular and Cellular Biology·K P MullaneB Schaffhausen
Feb 27, 1999·Genes & Development·R Losick, J Dworkin
Nov 5, 1999·In Vitro Cellular & Developmental Biology. Animal·A YenS Varvayanis

❮ Previous
Next ❯

Citations

Sep 5, 2001·Journal of Virology·O GjoerupT M Roberts
Mar 3, 2004·Molecular and Cellular Biology·Jianrong Wang, Andrew Yen
Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jianxuan ZhangPengbo Zhou
Sep 21, 2013·The Journal of Biological Chemistry·Justin H HwangBrian S Schaffhausen
Jan 22, 2004·Experimental Biology and Medicine·Satei Moqattash, John D Lutton

❮ Previous
Next ❯

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis