Polyomaviruses shedding in stool of patients with hematological disorders: detection analysis and study of the non-coding control region's genetic variability

Medical Microbiology and Immunology
Carla PreziosoV Pietropaolo

Abstract

Fragmented data are available on the human polyomaviruses (HPyVs) prevalence in the gastrointestinal tract. Rearrangements in the non-coding control region (NCCR) of JCPyV and BKPyV have been extensively studied and correlated to clinical outcome; instead, little information is available for KIPyV, WUPyV and MCPyV NCCRs. To get insights into the role of HPyVs in the gastrointestinal tract, we investigated JCPyV, BKPyV, KIPyV, WUPyV and MCPyV distribution among hematological patients in concomitance with gastrointestinal symptoms. In addition, NCCRs and VP1 sequences were examined to characterize the strains circulating among the enrolled patients. DNA was extracted from 62 stool samples and qPCR was carried out to detect and quantify JCPyV, BKPyV, KIPyV, WUPyV and MCPyV genomes. Positive samples were subsequently amplified and sequenced for NCCR and VP1 regions. A phylogenetic tree was constructed aligning the obtained VP1 sequences to a set of reference sequences. qPCR revealed low viral loads for all HPyVs searched. Mono and co-infections were detected. A significant correlation was found between gastrointestinal complications and KIPyV infection. Archetype-like NCCRs were found for JCPyV and BKPyV, and a high degree of NCCRs...Continue Reading

References

Jan 1, 1993·Journal of Medical Virology·L JinJ P Clewley
Jan 1, 1997·Archives of Virology·H T AgostiniG I Stoner
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·H T AgostiniG L Stoner
Apr 12, 2001·The Journal of General Virology·H T AgostiniG L Stoner
Jul 20, 2005·The Journal of Infectious Diseases·John A VanchiereJanet S Butel
Oct 10, 2006·Applied and Environmental Microbiology·Silvia Bofill-MasRosina Girones
Feb 9, 2007·Journal of Virology·Tobias AllanderBjörn Andersson
Aug 24, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S BialasiewiczT P Sloots
Jan 19, 2008·Science·Huichen FengPatrick S Moore
Apr 18, 2008·Briefings in Bioinformatics·Sudhir KumarKoichiro Tamura
May 27, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Cristina CostaRossana Cavallo
Jan 1, 2009·Emerging Infectious Diseases·Lili RenJianwei Wang
Jan 23, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Florian NeskeBenedikt Weissbrich
Jan 28, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marietjie VenterRia Lassauniere
May 26, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kalle KantolaKlaus Hedman
Jun 6, 2009·Journal of Clinical Microbiology·John A VanchiereJanet S Butel
Jun 12, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Seweryn BialasiewiczTheo P Sloots
Jul 7, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Muhammed Babakir-MinaMarco Ciotti
Jul 25, 2009·Journal of Medical Virology·Muhammed Babakir-MinaMarco Ciotti
Jul 31, 2009·Journal of Virology·Martyn K WhiteKamel Khalili
Aug 4, 2009·Journal of Virological Methods·Massimiliano BergalloCristina Costa
Sep 24, 2009·Journal of Medical Virology·Gunn-Hege OlsenChristine Hanssen Rinaldo
Jun 16, 2011·The Journal of Infectious Diseases·Carl E ReidJohn P Carulli
Mar 12, 2013·Nature Reviews. Microbiology·James A DeCaprio, Robert L Garcea
Oct 15, 2013·The Journal of Infection·Michelle Pinto, Simon Dobson
Oct 19, 2014·Food and Environmental Virology·P Di BonitoG La Rosa
Sep 1, 2015·Bioinformatics·Ulrich BodenhoferSepp Hochreiter
May 21, 2016·Journal of the Pediatric Infectious Diseases Society·John A VanchiereJanet S Butel
Nov 4, 2017·Viruses·Francois HelleGilles Duverlie

❮ Previous
Next ❯

Datasets Mentioned

BETA
EU375803
EF444549

Methods Mentioned

BETA
PCR

Software Mentioned

Molecular Evolutionary Genetics Analysis ( Mega )
ClustalW2

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.