Polyoxometalates function as indirect activators of a G protein-coupled receptor.

Metallomics : Integrated Biometal Science
Duaa AlthumairyDebbie C Crans

Abstract

The luteinizing hormone receptor (LHR), a G protein-coupled receptor (GPCRs), can initiate signaling in the presence of some vanadium-containing compounds as a result of vanadium compound interactions with the membrane lipids and/or the cell membrane lipid interface. The ability of LHR expressed in CHO cells to initiate signaling in the presence of highly charged and water-soluble polyoxovanadates (POV) including Na3[H3V10O28] (V10) and two mixed-valence heteropolyoxovanadates, K(NH4)4[H6V14O38(PO4)]·11H2O (V14) and [(CH3)4N]6[V15O36(Cl)] (V15), was investigated here. Interactions of the vanadium compounds with CHO cells decreased the packing of membrane lipids, drove aggregation of LHR and increased signal transduction by LHR. Cell responses were comparable to, or in the case of V14 and V15, greater than those seen for cells treated with human chorionic gonadotropin (hCG), a naturally-occurring LHR ligand produced in early pregnancy in humans. POV effects were observed for CHO cells where LHR was expressed at 10 000 or 32 000 LHR per cell but not when LHR was overexpressed with receptor numbers >100 000 LHR per cell. To determine which POV species were present in the cell medium during cell studies, the speciation of vanadate ...Continue Reading

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Citations

Feb 15, 2021·Journal of Inorganic Biochemistry·Kateryna KostenkovaDebbie C Crans
Apr 7, 2021·Journal of Inorganic Biochemistry·Kahoana PostalGiovana G Nunes
Jul 26, 2021·Current Opinion in Structural Biology·Jörg ReichenwallnerOliver P Ernst
Sep 16, 2021·Chemistry : a European Journal·Ibrahima Fa BambaEmmanuel Cadot
Dec 24, 2021·FEBS Letters·Diogo Henrique KitaGlaucio Valdameri

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Methods Mentioned

BETA
X-ray
Fluorescence
FRET
transfection
nuclear
electron paramagnetic resonance
NMR

Software Mentioned

MetaFluor
MetaMorph
R
Image J

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