PMID: 4894691Apr 1, 1969Paper

Polypeptide chain initiation in E. coli: isolation of homogeneous initiation factor E2 and its relation to ribosomal proteins

Proceedings of the National Academy of Sciences of the United States of America
Y B ChaeS Ochoa

Abstract

Previous work has shown that F(2), one of several ribosomal factors involved in polypeptide chain initiation, functions in the binding of formylmethionyl-transfer RNA (fMet approximately tRNA(f)) to a messenger RNA-ribosome complex. F(2) was isolated from 1.0 M ammonium chloride washes of E. coli Q13 ribosomes as a protein homogeneous on polyacrylamide gel electrophoresis at both pH 4.5 and 7.8. Its molecular weight is approximately 80,000. Comparison of electrophoretic patterns of ribosomal proteins from NH(4)Cl-washed and unwashed ribosomes and F(2), at pH 4.5, shows that F(2) corresponds to the slowest-moving component of the proteins derived from unwashed ribosomes. This component is missing from the NH(4)Cl-washed ribosomes. The activity of F(2) is stimulated by two additional factors, initiation factor F(1) and a factor(s) present in a narrow ammonium sulfate fraction of the ribosomal NH(4)Cl wash. The nature of the latter is unknown.

References

Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·Y Nishizuka, F Lipmann
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Gordon
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D KolakofskyR E Thach
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M SalasS Ochoa
May 1, 1965·Analytical Biochemistry·P H Duesberg, R R Rueckert
Mar 1, 1964·Biochemistry·D A YPHANTIS
Nov 1, 1964·Journal of Molecular Biology·J P WALLER

❮ Previous
Next ❯

Citations

Nov 1, 1968·Die Naturwissenschaften·S Ochoa
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R MazumderS Ochoa
Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·Y B ChaeS Ochoa
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff, S Ochoa
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J ModolellD Vazquez
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D A ShafritzW F Anderson
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff, S Ochoa
Mar 31, 1970·Biochimica Et Biophysica Acta·D L Weller, R E Sjogren
May 14, 1971·Journal of Molecular Biology·R M Young, D Nakada
Oct 14, 1971·Journal of Molecular Biology·H W KingJ J Shearman
Aug 1, 1977·Archives of Biochemistry and Biophysics·J W HersheyJ L Fakunding
Oct 5, 2007·Methods in Enzymology·Nicolas Locker, Peter J Lukavsky
Sep 1, 1971·Angewandte Chemie·G Schreiber
Feb 25, 1970·FEBS Letters·J AlbrechtL Bosch
May 1, 1972·FEBS Letters·M Grunberg-ManagoM Graffe
Oct 15, 1971·FEBS Letters·Rajarshi Mazumder
May 21, 1971·Nature·B D Davis
Jul 4, 1970·Nature·A L Beaudet, C T Caskey
Dec 26, 1970·Nature·S SabolS Ochoa
Feb 1, 1971·European Journal of Biochemistry·S Lee-HuangS Ochoa
Mar 1, 1973·European Journal of Biochemistry·S SabolS Ochoa
May 19, 1971·Nature: New Biology·P S RudlandB F Clark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.