PMID: 4899878Jul 1, 1969Paper

Polypeptide chain initiation in E. coli: studies on the function of initiation factor F1

Proceedings of the National Academy of Sciences of the United States of America
Y B ChaeS Ochoa

Abstract

The requirement of initiation factors F(1) (highly purified) and F(2) (electrophoretically homogeneous) for ribosomal binding of N-formylmethionyl transfer RNA (fMet approximately tRNA) at low Mg(2+) concentration (3.5 mM), with the trinucleoside diphosphate ApUpG as messenger, was studied under various experimental conditions with 30S + 50S ribosomes and with 30S subunits alone. The results were qualitatively the same in both cases but the amount of binding was two to three times higher when both 30S and 50S subunits were present. Although there was a virtually absolute requirement for F(2) in all cases, considerable binding occurred at 0 degrees in the absence of added F(1). F(1) addition stimulated binding up to twofold under these conditions. However, at 25 degrees , the temperature at which the reaction is usually carried out, there was very little binding with F(2) alone and addition of F(1) stimulated the reaction five- to sixfold. Contrary to current belief, the GTP analog 5'-guanylyldiphosphonate (GMP-PCP) cannot replace GTP in the binding reaction. In particular, there was but little stimulation of binding (about 1.5-fold) by addition of F(1) to F(2)-containing samples when GMP-PCP was used. In marked contrast, bindin...Continue Reading

References

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Mar 16, 1968·Nature·M A MukundanR E Thach
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·Z VogelD Elson
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Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·Y B ChaeS Ochoa
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Citations

Aug 1, 1976·Die Naturwissenschaften·S Ochoa
Mar 27, 1981·Molecular and Cellular Biochemistry·A Parmeggiani, G Sander
Dec 14, 1970·Biochimica Et Biophysica Acta·W Szer, Z Kurylo-Borowska
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff, S Ochoa
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D A ShafritzW F Anderson
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S S Thach, R E Thach
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D A ShafritzW F Anderson
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D A ShafritzW F Anderson
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff, S Ochoa
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P SarkarU Maitra
Aug 16, 1972·Biochimica Et Biophysica Acta·A C Kay, M Grunberg-Manago
May 10, 1972·Biochimica Et Biophysica Acta·R BenneH O Voorma
Oct 14, 1971·Journal of Molecular Biology·H FriedmanA Rich
Oct 14, 1971·Journal of Molecular Biology·H W KingJ J Shearman
Jun 28, 1972·Biochemical and Biophysical Research Communications·F Golini, R E Thach
Apr 1, 1972·Archives of Biochemistry and Biophysics·H WeissbachN Brot
Sep 1, 1971·Angewandte Chemie·G Schreiber
Apr 1, 1974·FEBS Letters·J Chu, R Mazumder
Oct 15, 1971·FEBS Letters·Rajarshi Mazumder
Dec 26, 1970·Nature·S SabolS Ochoa
May 1, 1972·European Journal of Biochemistry·J C LelongM Revel
Jun 15, 1976·European Journal of Biochemistry·G A van der HofstadH O Voorma
Feb 1, 1971·European Journal of Biochemistry·S Lee-HuangS Ochoa
Jan 15, 1973·European Journal of Biochemistry·R BenneH O Voorma
Oct 5, 1973·European Journal of Biochemistry·R BenneH O Voorma
Feb 17, 1971·Nature: New Biology·S S Thach, R E Thach

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