PMID: 4897026May 1, 1969Paper

Polypeptide chain initiation in E. coli: sulfhydryl groups and the function of initiation factor F2

Proceedings of the National Academy of Sciences of the United States of America
R MazumderS Ochoa

Abstract

The activity of the chain initiation factor F(2) in promoting the messenger-dependent binding of formylmethionyl-transfer RNA (fMet approximately tRNA(f)) to purified E. coli ribosomes is inhibited by sulfhydryl-binding reagents, such as N-ethylmaleimide or p-hydroxymercuribenzoate, but prior incubation with guanosine triphosphate (GTP) or with ribosomes largely prevents this inhibition. The effect of GTP suggests that it forms a complex with F(2) whereby "active" sulfhydryl groups become sheltered. Experiments on the time course of the binding reaction, with and without preincubation of F(2) with GTP, and gel filtration experiments with (3)H-labeled GTP lend support to this suggestion.

References

Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Gordon
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M B HilleA J Wahba
Nov 1, 1968·Die Naturwissenschaften·S Ochoa
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·Y B ChaeS Ochoa

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Citations

Aug 1, 1976·Die Naturwissenschaften·S Ochoa
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff, S Ochoa
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A H LockwoodU Maitra
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D A ShafritzW F Anderson
May 1, 1971·Archives of Biochemistry and Biophysics·D L MillerH Weissbach
Mar 5, 1973·Journal of Molecular Biology·Y Groner, M Revel
Feb 1, 1977·Toxicology and Applied Pharmacology·J M JacobsJ B Cavanagh
Nov 1, 1971·Archives of Biochemistry and Biophysics·J A Beeley
Aug 21, 1996·Biochimica Et Biophysica Acta·J A Willis, T Schleich
Nov 1, 1973·CRC Critical Reviews in Toxicology·R O Rechnagel, E A Glende
Sep 1, 1971·Angewandte Chemie·G Schreiber
Jun 15, 1972·FEBS Letters·M YoshidaB F Clark
Oct 15, 1971·FEBS Letters·Rajarshi Mazumder
Dec 26, 1970·Nature·S SabolS Ochoa
Jul 1, 1977·European Journal of Biochemistry·G A van der HofstadH O Voorma
May 19, 1971·Nature: New Biology·P S RudlandB F Clark
Feb 17, 1971·Nature: New Biology·S S Thach, R E Thach

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