PMID: 12754287May 20, 2003Paper

Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I expression in gastric carcinoma cell lines

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Nuno T MarcosCelso A Reis

Abstract

Mucin O-glycosylation in cancer is characterized by aberrant expression of immature carbohydrate structures leading to exposure of simple mucin-type carbohydrate antigens and peptide epitopes. Glycosyltransferases controlling the initial steps of mucin O-glycosylation are responsible for the altered glycosylation observed in cancer. We studied the expression in gastric cell lines of six UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-T1, T2, T3, T4, T6, T11) that catalyze the initial key step in the regulation of mucin O-glycosylation, the transfer of GalNAc from UDP-GalNAc to serine and threonine residues. We also studied the expression of ST6GalNAc-I, the enzyme responsible for the synthesis of Sialyl-Tn antigen (NeuAcalpha2,6GalNAc) and the ST3Gal-I, the enzyme responsible for the synthesis of Sialyl-T antigen (NeuAcalpha2,3Galbeta1,3GalNAc). This study was done using specific monoclonal antibodies, enzymatic assays, and RT-PCR. Our results showed that GalNAc-T1, -T2, and -T3 have an ubiquitous expression in all gastric cell lines, whereas GalNAc-T4, -T6, and -T11 show a restricted expression pattern. The immunoreactivity with MAb VU-2-G7 suggests that, apart from GalNAc-T4, another GalNAc transferase is in...Continue Reading

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Citations

Aug 7, 2012·Cellular and Molecular Life Sciences : CMLS·Milada Stuchlová HorynováJan Novak
Apr 27, 2007·Nature·Danica P Galonić, David Y Gin
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daniel AmbortGunnar C Hansson
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Oct 16, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joana GomesCelso A Reis
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May 1, 2021·European Journal of Pharmacology·Iwona RadziejewskaAnna Bielawska

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