Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tn1 and Tn3.

Journal of Bacteriology
G DouganD J Sherratt

Abstract

Escherichia coli K-12 minicells were employed to examine polypeptides encoded by plasmids carrying wild-type and mutant Tn1 or Tn3 transposition elements. Tn1- and Tn3-containing minicells express high levels of four transposon-specified polypeptides. Three, of molecular weights 30,000, 28,000, and 25,000, are related immunologically to beta-lactamase, the enzyme responsible for ampicillin hydrolysis. A fourth polypeptide of molecular weight 19,000 is encoded by the Tn1 or Tn3 region which spans the BamHI cleavage site. Mutant transposons which no longer produce this polypeptide transpose at higher than wild-type frequencies to give aberrant transposition products (Gill et al., J. Bacteriol. 136: 742--756, 1978; Heffron et al., Proc. Natl. Acad. Sci U.S.A. 72:3632--3627, 1975). No expression could be detected from a region of the transposons extending from the inverted repeat sequence distal to the beta-lactamase gene to more than half the distance into the Tn1 or Tn3 sequence.

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Citations

Oct 1, 1979·Molecular & General Genetics : MGG·A Arthur, D Sherratt
Jan 1, 1984·Molecular & General Genetics : MGG·T OlschlägerV Braun
Jan 1, 1980·Microbiology and Immunology·T YamamotoS Yamagishi
Oct 1, 1986·Molecular & General Genetics : MGG·K A ArmstrongE Ohtsubo
Jan 1, 1980·Microbiology and Immunology·T YamamotoS Yamagishi
Sep 1, 1980·Journal of Bacteriology·D A Johnson, N S Willetts
May 1, 1980·Journal of Bacteriology·S J Chiang, R C Clowes
Apr 1, 1985·Journal of Bacteriology·D O Clegg, D E Koshland
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Jan 5, 1981·Journal of Molecular Biology·M Giphart-GasslerP van de Putte
Nov 30, 1981·Biochemical and Biophysical Research Communications·A BollenA Herzog
May 1, 1980·Plasmid·P Starlinger

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