Polypeptides of hepatitis B surface antigen produced in transgenic potato

Gene
P EhsaniN N Domansky

Abstract

Chimeric genes containing the genomic fragments of hepatitis B virus (HBV) coding for middle (M) and major/small (S) surface proteins were constructed and expressed in transgenic potato plants under the control of the cauliflower mosaic virus 35S promoter. Enzyme-linked immunosorbent assay (ELISA) revealed the presence of HBV surface antigen (HBsAg) determinants in extracts from transgenic plants carrying both constructions, the pre-S2 determinant was identified using the corresponding monoclonal antibodies (mAb) in a dot immunobinding assay. Polypeptides of the immunoaffinity-purified S and M proteins were analyzed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. Two polypeptides of the M protein, probably representing different glycosylated forms, and the multimeric forms of HBsAg were observed.

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H S MasonC J Arntzen
Oct 10, 1985·Nature·P TiollaisA Dejean
Nov 26, 1984·Nucleic Acids Research·M Bevan
Jul 15, 1983·Journal of Immunological Methods·F C Bennett, L C Yeoman
Sep 1, 1995·Trends in Biotechnology·H S Mason, C J Arntzen
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y ThanavalaC Arntzen

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Citations

Jun 5, 2003·International Journal for Parasitology·Stephen J Streatfield, John A Howard
Feb 7, 2001·Current Opinion in Biotechnology·A M Walmsley, C J Arntzen
May 10, 2005·Immunology and Cell Biology·Stephen J Streatfield
Jan 23, 2013·International Journal of Molecular Sciences·Tomasz Pniewski
May 21, 2015·Pharmaceutical Biology·Sara MohammadzadehParastoo Ehsani
Apr 10, 2009·Biotechnology Advances·Siddharth TiwariRakesh Tuli
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Luca SantiHugh Mason
Aug 2, 2003·Biochemical and Biophysical Research Communications·Rodolfo ValdésCarlos Borroto
Apr 13, 2006·Expert Review of Vaccines·Yasmin ThanavalaHugh S Mason
Oct 7, 2010·Expert Review of Vaccines·António RoldãoPaula M Alves
Mar 11, 2005·Expert Opinion on Emerging Drugs·Richard M TwymanRainer Fischer
Jul 5, 2005·Expert Opinion on Investigational Drugs·P B RodgersJ R Adair
Feb 23, 2007·Clinical and Vaccine Immunology : CVI·Xiao-Ming LouHua-Kun Wang
Jan 1, 2007·Russian Journal of Plant Physiology : a Comprehensive Russian Journal on Modern Phytophysiology·E B RukavtsovaYa I Bur'yanov

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