Polypeptides of the maize amyloplast stroma. Stromal localization of starch-biosynthetic enzymes and identification of an 81-kilodalton amyloplast stromal heat-shock cognate

Plant Physiology
Y YuB P Wasserman

Abstract

In the developing endosperm of monocotyledonous plants, starch granules are synthesized and deposited within the amyloplast. A soluble stromal fraction was isolated from amyloplasts of immature maize (Zea mays L.) endosperm and analyzed for enzyme activities and polypeptide content. Specific activities of starch synthase and starch-branching enzyme (SBE), but not the cytosolic marker alcohol dehydrogenase, were strongly enhanced in soluble amyloplast stromal fractions relative to soluble extracts obtained from homogenized kernels or endosperms. Immunoblot analysis demonstrated that starch synthase I, SBEIIb, and sugary1, the putative starch-debranching enzyme, were each highly enriched in the amyloplast stroma, providing direct evidence for the localization of starch-biosynthetic enzymes within this compartment. Analysis of maize mutants shows the deficiency of the 85-kD SBEIIb polypeptide in the stroma of amylose extender cultivars and that the dull mutant lacks a >220-kD stromal polypeptide. The stromal fraction is distinguished by differential enrichment of a characteristic group of previously undocumented polypeptides. N-terminal sequence analysis revealed that an abundant 81-kD stromal polypeptide is a member of the Hsp70 ...Continue Reading

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Citations

May 22, 2001·Archives of Biochemistry and Biophysics·H H MuG M Carman
Sep 18, 2002·Archives of Biochemistry and Biophysics·Chunyuan WuMartha G James
Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·P D Commuri, P L Keeling
Mar 12, 2008·The FEBS Journal·Allan StensballeKaren G Welinder
Feb 12, 2010·The Biochemical Journal·Xiaoping FuGuanghua Zhao
Jul 12, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jixun LuoZhongyi Li
Oct 10, 1998·Plant Physiology·Z P ZhuA M Smith

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