Polyphenol oxidase activity in subcellular fractions of tall fescue contaminated by polycyclic aromatic hydrocarbons

Journal of Environmental Quality
Wanting LingYandi Sun

Abstract

Understanding enzyme responses to contamination with persistent organic pollutants (POPs) is a key step in the elucidation of POP metabolic mechanisms in plants. However, there is little information available on enzyme activity in subcellular fractions of POP-contaminated plants. To our knowledge, this is the first study to investigate the activities of polyphenol oxidase (PPO) in cell fractions of plants under contamination stress from polycyclic aromatic hydrocarbons (PAHs) using a greenhouse batch technique. Three parameters, E(cell), E(cell-n), and P(cell), denoting the amount of PPO activity, cell fraction content-normalized PPO activity, and proportion of PPO activity in each cell fraction, respectively, were used in this study. Contamination with phenanthrene, as a representative PAH, at a relatively high level (>0.23 mg L⁻¹) in culture solution generally stimulated PPO activity in tall fescue (Festuca arundinacea Schreb.) roots and shoots and their cellular fractions. The amount and distribution proportion of PPO activity in each cell fraction of phenanthrene-contaminated roots and shoots were (in descending order): cell solution > > cell wall > cell organelles. Cell solution was the dominant storage domain of PPO activ...Continue Reading

References

Jun 1, 1997·Ecotoxicology and Environmental Safety·D UgrekhelidzeG Kvesitadze
Aug 7, 2003·Environmental Geochemistry and Health·C A BradyP T Lynch
Jun 15, 2005·Environmental Science & Technology·Edward WildKevin C Jones
Aug 2, 2005·Environmental Science & Technology·Hui LiOuyong Xu
Jan 26, 2006·Environmental Science & Technology·Chris CollinsAlbania Grosso
Mar 1, 1980·Plant Physiology·H J Weigel, H J Jäger
Jul 6, 2006·Journal of Agricultural and Food Chemistry·Asmae AajoudPatrick Ravanel
Sep 15, 2009·Environmental Science & Technology·Yanzheng Gao, C D Collins
Apr 28, 2011·Journal of Environmental Quality·Yanzheng GaoZhaoxia Cheng
Jun 24, 2011·Environmental Science & Technology·Scott J GreenwoodBarbara A Zeeb
May 1, 1994·Environmental Science & Technology·S L Simonich, R A Hites

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