Polyphenolic extract isolated from Korean Lonicera japonica Thunb. induce G2/M cell cycle arrest and apoptosis in HepG2 cells: involvements of PI3K/Akt and MAPKs

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Hyeon-Soo ParkGon-Sup Kim

Abstract

Lonicera japonica Thunb. (L. japonica T.) has been used in Korean traditional medicine for long time because of its anti-cancer and hepatic protective effect. In this study, we investigated polyphenolic extract in L. japonica T. using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and its anti-cancer effect on hepatocarcinoma cells. Human HepG2 cell line was treated with various concentrations of polyphenolic extract. Apoptosis was detective by cell morphology, cell cycle analysis and immunoblot analysis. Polyphenolic extract inhibited cell proliferation at 48h in a dose-dependent manner. Polyphenolic extract affected HepG2 cell viability by inhibiting cell cycle progression at the G2/M transition and inducing apoptosis. Polyphenolic extract also decreased the expression of CDK1, CDC25C, cyclin B1, pro-caspases-3 and -9 and poly ADP ribose polymerase, and affected the levels of mitochondrial apoptotic-related proteins. The phosphorylation of extracellular signal-related kinase ½ (ERK 1/2), c-Jun N-terminal kinase (JNK), and p-38 mitogen-activated protein kinases (MAPKs) were increased in HepG2 cells treated with polyphenolic extract, whereas Akt was dephosphorylated. These results indi...Continue Reading

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Citations

Sep 24, 2015·International Journal of Molecular Sciences·Venu Venkatarame Gowda SaralammaGon Sup Kim
Jul 16, 2015·Phytotherapy Research : PTR·Jae Min HanWoong Mo Yang
Dec 3, 2014·Environmental Toxicology and Pharmacology·Ping JiangZheng-tao Wang
Nov 12, 2013·Journal of Ethnopharmacology·Yang-Chun ParkYoon Yeo
Aug 11, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yujie LiHainan Wang
Oct 16, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yan QiaoXuebo Liu
Jun 17, 2014·Journal of Asian Natural Products Research·Hua YangGuang-Zhong Yang
Mar 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miao YuYujun Liu
Jul 16, 2019·Phytochemical Analysis : PCA·Nataša NastićAntonio Segura-Carretero
Oct 10, 2019·Journal of the Science of Food and Agriculture·Rong-Jiao LiWei-Xi Li
Mar 25, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yuke LiQiang Fu
Aug 10, 2021·ACS Omega·Ahmed E FazaryAly F Mohamed

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