Polyphenols-rich extract from Pleurotus eryngii with growth inhibitory of HCT116 colon cancer cells and anti-inflammatory function in RAW264.7 cells

Food & Function
Qiuhui HuWenjian Yang

Abstract

Edible mushrooms are rich sources of bioactive components. In this study, a polyphenol-rich extract, designated as PPEP, was isolated from an edible mushroom, Pleurotus eryngii. Using ultra high performance liquid chromatograph combined with triple time-of-flight mass spectrometry (UPLC-TOF/MS/MS), gallic acid monohydrate, 3-(3,4-dihydroxyphenyl)-propionic acid, methyl gallate, syringic acid, ellagic acid and catechin were identified in PPEP. This phenolic-rich extract PPEP exhibited anti-inflammatory effect in lipopolysaccharide-stimulated RAW264.7 macrophages by inhibiting the overproduction of pro-inflammatory mediators including nitric oxide (NO) and reactive oxygen species (ROS). It was demonstrated that the anti-inflammatory effects of PPEP were associated with the inhibition of iNOS expression, suppression of p-IκB protein expression and inhibition of NF-κB and IκB mRNA expression. Next, the inhibitory effect of PPEP against human colon cancer cells was also determined. PPEP suppressed cell proliferation of human colon cancer HCT116 cells in a dose- and time-dependent fashion, while it showed no inhibitory effect on normal human colonic myofibroblasts CCD-18Co cells at the same tested concentrations (0-200 μg mL-1). More...Continue Reading

References

Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
May 20, 1999·Annual Review of Pharmacology and Toxicology·D G Johnson, C L Walker
Mar 5, 2002·Nature Medicine·Tzong-Shyuan Lee, Lee-Young Chau
Aug 16, 2005·Molecular Immunology·Aarne FleischerAngelita Rebollo
Feb 1, 2008·Inflammopharmacology·J N SharmaS S Parvathy
Apr 28, 2010·Gastroenterology·Janos TerzićMichael Karin
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal
Dec 4, 2010·Journal of Agricultural and Food Chemistry·Emilio Alvarez-ParrillaFereidoon Shahidi
Mar 4, 2011·Journal of Agricultural and Food Chemistry·Fernando Sánchez-PatánBegoña Bartolomé
Jul 21, 2011·Nature Immunology·Yinon Ben-Neriah, Michael Karin
Sep 29, 2011·Journal of Experimental & Clinical Cancer Research : CR·Rebecca S Y Wong
Oct 26, 2013·Bioorganic & Medicinal Chemistry Letters·Yanqin LiuJohn A Carver
Mar 22, 2014·Journal of Ethnopharmacology·Alfred Mugambi MarigaQiu-hui Hu
Mar 22, 2014·Journal of the Science of Food and Agriculture·Alfred Mugambi MarigaQiuhui Hu
Apr 8, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katheryn M Goodrich, Andrew P Neilson
Feb 28, 2015·Food Chemistry·Farid KhalloukiRobert W Owen
Jan 16, 2016·Journal of Functional Foods·Xian WuHang Xiao
May 25, 2016·Journal of Agricultural and Food Chemistry·Mingyue SongHang Xiao
Jul 20, 2017·Nature Immunology·Laurence ZitvogelGuido Kroemer
Nov 5, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Varinder KaurSatwinderjeet Kaur
Nov 9, 2017·Cancer Letters·Shireen ChikaraSharad S Singhal

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Citations

May 28, 2019·Molecular Nutrition & Food Research·Qiuhui HuHang Xiao
Apr 30, 2019·Frontiers in Immunology·Anna Maria MileoStefania Miccadei
Feb 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao XuMingsheng Dong
Oct 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying BaiHang Qi
Feb 2, 2021·Journal of Traditional and Complementary Medicine·Panthakarn RangsinthSiriporn Chuchawankul
Feb 26, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Juan XuXueQian Wu
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dinesh K PatelKi-Taek Lim
Sep 1, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yanchu LiXianyong Li

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Methods Mentioned

BETA
electrophoresis
flow cytometry

Software Mentioned

Reaxy
Scifinder
MassLynx

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