Polyphyllin D induces apoptosis in human erythrocytes through Ca²⁺ rise and membrane permeabilization

Archives of Toxicology
Minghui GaoS K Kong

Abstract

Polyphyllin D (PD) is a potent anticancer agent isolated from a traditional medicinal herb Paris polyphylla that has been used in China for many years to treat cancer. PD is not a substrate of p-glycoprotein, and it can bypass the multi-drug resistance in cancer cell line R-HepG2. However, the effect of PD on the induction of cell death in human erythrocytes remains unknown. Given that PD is a small molecule that can depolarize the mitochondrial membrane potential and release apoptosis-inducing factor (AIF) in isolated mitochondria, we hypothesized that the apoptogenic effect of PD in human erythrocytes devoid of mitochondria would be minimal. This study therefore tried to evaluate the in vitro effect of PD on hemolysis and apoptosis in human erythrocytes. Apoptosis in human red blood cells (RBCs), also known as eryptosis or erythroptosis, after PD treatment was determined by flow cytometry and confocal microscopy for the phosphatidyl-serine externalization and other apoptosis feature events. False to our prediction, PD caused hemolysis and eryptosis/erythroptosis in human RBCs. Mechanistically, elevation in the cytosolic Ca²⁺ ion level seems to be a key but not the only mediator in the PD-mediated eryptosis/erythroptosis becau...Continue Reading

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Citations

Mar 22, 2013·Archives of Toxicology·Kashif Jilani, Florian Lang
Nov 10, 2013·Archives of Toxicology·T GebelJ G Hengstler
Jun 27, 2013·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Florian LangMichael Föller
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Jul 19, 2013·Toxins·Kashif JilaniFlorian Lang
Sep 13, 2013·Toxins·Adrian LupescuFlorian Lang
Oct 25, 2013·Toxins·Elisabeth LangFlorian Lang
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Oct 29, 2014·Basic & Clinical Pharmacology & Toxicology·Salvatrice CalabròFlorian Lang
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Dec 25, 2019·Chemico-biological Interactions·Ye TianMei-Yun Fang

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