Polyploid Adipose Stem Cells Shift the Balance of IGF1/IGFBP2 to Promote the Growth of Breast Cancer

Frontiers in Oncology
Roberta Fajka-BojaRobert L Katona

Abstract

Background: The close proximity of adipose tissue and mammary epithelium predispose involvement of adipose cells in breast cancer development. Adipose-tissue stem cells (ASCs) contribute to tumor stroma and promote growth of cancer cells. In our previous study, we have shown that murine ASCs, which undergo polyploidization during their prolonged in vitro culturing, enhanced the proliferation of 4T1 murine breast cancer cells in IGF1 dependent manner. Aims: In the present study, our aim was to clarify the regulation of ASC-derived IGF1. Methods: 4T1 murine breast carcinoma cells were co-transplanted with visceral fat-derived ASCs (vASC) or with the polyploid ASC.B6 cell line into female BALB/c mice and tumor growth and lung metastasis were monitored. The conditioned media of vASCs and ASC.B6 cells were subjected to LC-MS/MS analysis and the production of IGFBP2 was verified by Western blotting. The regulatory effect was examined by adding recombinant IGFBP2 to the co-culture of ASC.B6 and 4T1. Akt/protein kinase B (PKB) activation was detected by Western blotting. Results: Polyploid ASCs promoted the tumor growth and metastasis more potently than vASCs with normal karyotype. vASCs produced the IGF1 regulator IGFBP2, which inhibi...Continue Reading

References

Jun 3, 2004·Journal of Molecular Endocrinology·B S SchüttM W Elmlinger
Mar 26, 2005·Journal of Clinical Pathology·L-T BusundH Stalsberg
Nov 17, 2006·Cancer Research·Yi Fu ZhouGregg L Semenza
Dec 25, 2009·Blood·Karin TarteUNKNOWN Société Française de Greffe de Moelle et Thérapie Cellulaire
Jan 28, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicole M Probst-HenschHolger Moch
Sep 9, 2010·Journal of Mammary Gland Biology and Neoplasia·Erin J McCaveBrian W Booth
Oct 8, 2013·Cytotherapy·Marieke Roemeling-van RhijnMartin J Hoogduijn
Jan 27, 2015·Journal of Cell Communication and Signaling·Steven W YauVincenzo C Russo
Mar 10, 2015·Molecular Cancer·Panagiotis F ChristopoulosMichael Koutsilieris
Mar 17, 2015·Frontiers in Endocrinology·Adam Pickard, Dennis J McCance
May 23, 2015·Stem Cells International·Riccardo SchweizerJan A Plock
Jun 25, 2015·Journal of Cancer Epidemiology·Chelsea CatsburgThomas E Rohan
Dec 14, 2016·The New England Journal of Medicine·Peter W MarksRobert M Califf
Apr 22, 2017·Cell·Brendan D Manning, Alex Toker
Nov 21, 2017·NPJ Precision Oncology·Roudy Chiminch Ekyalongo, Douglas Yee
Dec 20, 2017·Journal of Molecular Endocrinology·L A Bach
Mar 15, 2018·Journal of Molecular Endocrinology·Fumihiko Hakuno, Shin-Ichiro Takahashi
Jun 14, 2019·Stem Cells International·Mimmi PatrikoskiSusanna Miettinen
Jul 5, 2019·Stem Cells·Rachel A SabolBruce A Bunnell

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Citations

May 1, 2021·International Journal of Molecular Sciences·Francesca PiccottiMarta Truffi

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Methods Mentioned

BETA
FCS
xenograft

Clinical Trials Mentioned

NCT02780401

Software Mentioned

ProteinProspector
EXCEL
PAVA script

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