PMID: 20647776Jul 22, 2010Paper

Polyploid megakaryocytes can complete cytokinesis

Cell Cycle
Younes Leysi-DerilouNicolas Pineault

Abstract

Megakaryocytes (MK) undergo polyploidization through endomitosis, a mitotic process that ends prematurely due to aborted cytokinesis. To better understand this and other events associated with MK differentiation, we performed long-term and large-field live cell imaging of human MKs derived in cord blood (CB) and bone marrow (BM) CD34(+) cell cultures. Polyploid level of imaged cells was evaluated using three complementary approaches; cell history, cell size and ploidy correlation and nuclei staining. This system and strategy enabled the direct observation of the development of a large number of MKs (n=4865) and to quantify their fates. The most significant finding of this study is that a considerable proportion of polyploid MKs could complete cytokinesis. This unexpected process gave rise to polyploid daughter cell(s) with normal fates and contributed significantly to the expansion of polyploid MKs. Further analyses revealed that the proliferation rate amongst polyploid MKs was inversely correlated to their ploidy level, and that this phenomenon was much more frequent in CB- than BM-derived MKs. Accordingly, endomitosis was identified as the dominant fate of polyploid BM-MKs, while this was less accentuated for polyploid CB-MKs...Continue Reading

Citations

Mar 6, 2012·Differentiation; Research in Biological Diversity·Younes Leysi-DerilouNicolas Pineault
Dec 7, 2011·Transfusion·Nicolas Pineault, Lucie Boyer
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·P Karagiannis, K Eto
Oct 26, 2011·Experimental Hematology·Pani A ApostolidisEleftherios T Papoutsakis
Nov 20, 2012·Cell Cycle·Larissa LordierYunhua Chang
Oct 25, 2013·Blood·Anita EcklyChristian Gachet
Mar 7, 2019·PLoS Computational Biology·Yuri Kheifetz, Markus Scholz
Apr 16, 2011·Blood·Nicolas Pineault
Sep 24, 2019·Platelets·William Vainchenker, Hana Raslova
Aug 22, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Diane S Krause, John D Crispino

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