Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa

Evidence-based Complementary and Alternative Medicine : ECAM
Izabela Barbosa MoraesFoued Salmen Espindola

Abstract

Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins. Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM. Despite its popularity, there is little information about its effect on hepatic tissue. Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV). Histology was determined by fixing the livers, processing, and staining with HE. Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA). Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry. In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased. AEV treatment restored all parameters to normal levels. The oxidative stress analysis of hepatic mitochondria fraction showed similar results. Lower polyploid cell populations were found in the diabetic rat livers, ev...Continue Reading

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Citations

Nov 22, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Marcela Soto-GarcíaNorma Paniagua-Castro
Sep 21, 2017·Medicines·Neire Moura de GouveiaOlga M Palomino

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Methods Mentioned

BETA
glycosylation
flow cytometry
MDA

Software Mentioned

Image J
LEICA Application Suite
Minitab
SigmaStat

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